ArticleJournal of extracellular vesicles2024
Thermotolerance in S. cerevisiae as a model to study extracellular vesicle biology.
Article in Journal of extracellular vesicles, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Advances in Biological Functions and Applications of Feeding Microorganism-derived Extracellular Vesicles.Probiotics and antimicrobial proteins · 2026Review
- Engineering Yeast Extracellular Vesicle Biogenesis Through Rewiring Membrane Trafficking Pathways.Microbial biotechnology · 2026Article
- Yeast as a Model for Human Disease.International journal of molecular sciences · 2026Review
- Extracellular vesicle-mediated immunomodulation and targeted delivery: breakthroughs and challenges in rheumatoid arthritis therapy.Frontiers in immunology · 2026Review
- Selective DNA encapsulation in extracellular vesicles ofExtracellular vesicles and circulating nucleic acids · 2026Article
- Establishment of an Isolation System for Extracellular Vesicles ofJournal of fungi (Basel, Switzerland) · 2025Article
- Review
- Generalizability of Enzyme-Based Isolation Approach for Extracellular Vesicles From Traditional Medicinal Plants.Journal of extracellular biology · 2025Article
- Protein Biosynthesis and Carbon Catabolite Repression Are Transcriptionally Upregulated in Saccharomyces cerevisiae by Extracellular Fractions From Several Wine Yeast Species.Microbial biotechnology · 2025Article
- [Isolation and proteomic analysis of bacterial outer membrane vesicle subpopulations].Se pu = Chinese journal of chromatography · 2025Article
- Thermotolerant yeasts promoting climate-resilient bioproduction.FEMS yeast research · 2025Review
- Impact of Culture Duration on the Properties and Functionality of Yeast-Derived Extracellular Vesicles.Biomaterials research · 2025Article
- Exosome-mediated miRNA delivery: a molecular switch for reshaping neuropathic pain therapy.Frontiers in molecular neuroscience · 2025Review
- Review
- Fungal Extracellular Vesicle Proteins with Potential in Biological Interaction.Molecules (Basel, Switzerland) · 2024Review
- Thermotolerance in S. cerevisiae as a model to study extracellular vesicle biology.Journal of extracellular vesicles · 2024Article
- Immunomodulatory effects of immune cell-derived extracellular vesicles in melanoma.Frontiers in immunology · 2024Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The budding yeast Saccharomyces cerevisiae is a proven model organism for elucidating conserved eukaryotic biology, but to date its extracellular vesicle (EV) biology is understudied. Here, we show yeast transmit information through the extracellular medium that increases survival when confronted with heat stress and demonstrate the EV-enriched samples mediate this thermotolerance transfer. These samples contain vesicle-like particles that are exosome-sized and disrupting exosome biogenesis by targeting endosomal sorting complexes required for transport (ESCRT) machinery inhibits thermotolerance transfer. We find that Bro1, the yeast ortholog of the human exosome biomarker ALIX, is present in EV samples, and use Bro1 tagged with green fluorescent protein (GFP) to track EV release and uptake by endocytosis. Proteomics analysis reveals that heat shock protein 70 (HSP70) family proteins are enriched in EV samples that provide thermotolerance. We confirm the presence of the HSP70 ortholog stress-seventy subunit A2 (Ssa2) in EV samples and find that mutant yeast cells lacking SSA2 produce EVs but they fail to transfer thermotolerance. We conclude that Ssa2 within exosomes shared between yeast cells contributes to thermotolerance. Through this work, we advance Saccharomyces cerevisiae as an emerging model organism for elucidating molecular details of eukaryotic EV biology and establish a role for exosomes in heat stress and proteostasis that seems to be evolutionarily conserved.
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Registered trials
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