ArticleCommunications biology2024
Synthetic phage-based approach for sensitive and specific detection of Escherichia coli O157.
Article in Communications biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Article
- Isolation and characterization of bacteriophages for carbapenem resistant Enterobacter cloacae complex strains.Scientific reports · 2025Article
- Isolation and characterization of novel bacteriophages targeting Stenotrophomonas maltophilia.Scientific reports · 2025Article
- Synthetic and Functional Engineering of Bacteriophages: Approaches for Tailored Bactericidal, Diagnostic, and Delivery Platforms.Molecules (Basel, Switzerland) · 2025Review
- Development of a nonreplicative phage-based DNA delivery system and its application to antimicrobial therapies.PNAS nexus · 2025Article
- Phage engineering to overcome bacterial Tmn immunity in Dhillonvirus.Communications biology · 2025Article
- A Comprehensive Review on Phage Therapy and Phage-Based Drug Development.Antibiotics (Basel, Switzerland) · 2024Review
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Authors and funding
15 authors.
Funding
Abstract
Escherichia coli O157 can cause foodborne outbreaks, with infection leading to severe disease such as hemolytic-uremic syndrome. Although phage-based detection methods for E. coli O157 are being explored, research on their specificity with clinical isolates is lacking. Here, we describe an in vitro assembly-based synthesis of vB_Eco4M-7, an O157 antigen-specific phage with a 68-kb genome, and its use as a proof of concept for E. coli O157 detection. Linking the detection tag to the C-terminus of the tail fiber protein, gp27 produces the greatest detection sensitivity of the 20 insertions sites tested. The constructed phage detects all 53 diverse clinical isolates of E. coli O157, clearly distinguishing them from 35 clinical isolates of non-O157 Shiga toxin-producing E. coli. Our efficient phage synthesis methods can be applied to other pathogenic bacteria for a variety of applications, including phage-based detection and phage therapy.
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Registered trials
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