Evidence map›Paper›PMID 38710129›Full record

ArticleVirology2024

Commercial human 3D corneal epithelial equivalents for modeling epithelial infection in herpes keratitis.

Tatiana Borodianskiy-Shteinberg, Punam Bisht, Biswajit Das, Paul R Kinchington, Ronald S Goldstein

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Tatiana Borodianskiy-ShteinbergMina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900, Israel.
Punam BishtMina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900, Israel.
Biswajit DasMina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900, Israel.
Paul R KinchingtonDepartments of Ophthalmology and of Molecular Microbiology and Genetics, University of Pittsburgh, Pittsburgh, PA, 15213, USA.
Ronald S GoldsteinMina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900, Israel. Electronic address: ron.goldstein@biu.ac.il.

Funding

Virus Production and Manipulation of Protein/Gene Expression ModuleP30EY008098 · NEI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Ethan A Rossi · 1989 to 2026
$17.8M
Molecular studies of VZV infection, latency and reactivation in human neurons in-vitroR01AI122640 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI KINCHINGTON, PAUL R. · 2016 to 2020
$2.3M
Small non-coding RNAs of VZV: role in lytic and latent infectionR21AI156527 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI GOLDSTEIN, RONALD, KINCHINGTON, PAUL R. · 2021 to 2022
$393k
NEI NIH HHS P30 EY008098NIAID NIH HHS R01 AI122640NIAID NIH HHS R21 AI156527
6 · The paper itself

Abstract

Herpes stromal keratitis is the leading cause of infectious blindness in the western world. Infection by HSV1 is most common, but VZV and hCMV also infect the cornea. Multiple models of HSV1 corneal infection exist, but none for VZV and hCMV because of their host specificity. Here, we used commercially available 3D human corneal epithelial equivalents (HCEE) to study infection by these herpesviruses. HCEE was infected by HSV-1 and hCMV without requiring scarification and resulted in spreading infections. Spread of HSV-1 infection was rapid, while that of hCMV was slow. In contrast, infections with VZV required damage to the HCEE and did not spread. Acyclovir dramatically reduced replication of HSV-1 in this model. We conclude that highly quality-controlled, readily available HCEE is a useful model to study human-restricted herpesvirus infection of the human corneal epithelium and for screening of antiviral drugs for treating HSK in an 3D model system.

Indexed as

Antiviral AgentsEpithelium, CornealHerpesvirus 1, HumanKeratitis, HerpeticAcyclovirCytomegalovirusEpithelial CellsHerpesvirus 3, HumanHumansModels, BiologicalVirus ReplicationAcyclovirAntiviral Agents3D cultureDrug testingHerpes keratitisHerpesvirusHerpes zoster ophthalmicus

Identifiers

PMID38710129
PMCPMC12107712

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.