A Magnetic Pincher for the Dynamic Measurement of the Actin Cortex Thickness in Live Cells.

Joseph Vermeil, Valentin Laplaud, Anumita Jawahar, Dulamkhuu Bujaa, Damien Cuvelier, Julien Heuvingh, Olivia du Roure, Matthieu Piel

Abstract read

PubMed Publisher

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Joseph VermeilPhysique et Mécanique des Milieux Hétérogènes, ESPCI Paris, PSL University, CNRS, Univ Paris, Sorbonne Université, Paris, France.

Valentin LaplaudLadHyX, CNRS, Ecole polytechnique, Institut Polytechnique de Paris, Palaiseau, France.

Anumita JawaharPhysique et Mécanique des Milieux Hétérogènes, ESPCI Paris, PSL University, CNRS, Univ Paris, Sorbonne Université, Paris, France.

Dulamkhuu BujaaPhysique et Mécanique des Milieux Hétérogènes, ESPCI Paris, PSL University, CNRS, Univ Paris, Sorbonne Université, Paris, France.

Damien CuvelierUMR 144, Institut Curie and Institut Pierre Gilles de Gennes, PSL University, CNRS, Paris, France.

Julien HeuvinghPhysique et Mécanique des Milieux Hétérogènes, ESPCI Paris, PSL University, CNRS, Univ Paris, Sorbonne Université, Paris, France.

Olivia du RourePhysique et Mécanique des Milieux Hétérogènes, ESPCI Paris, PSL University, CNRS, Univ Paris, Sorbonne Université, Paris, France.

Matthieu PielUMR 144, Institut Curie and Institut Pierre Gilles de Gennes, PSL University, CNRS, Paris, France. matthieu.piel@curie.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The actin cortex is an essential element of the cytoskeleton allowing cells to control and modify their shape. It is involved in cell division and migration. However, probing precisely the physical properties of the actin cortex has proved to be challenging: it is a thin and dynamic material, and its location in the cell-directly under the plasma membrane-makes it difficult to study with standard light microscopy and cell mechanics techniques. In this chapter, we present a novel protocol to probe dynamically the thickness of the cortex and its fluctuations using superparamagnetic microbeads in a uniform magnetic field. A bead ingested by the cell and another outside the cell attract each other due to dipolar forces. By tracking their position with nanometer precision, one can measure the thickness of the cortex pinched between two beads and monitor its evolution in time. We first present the set of elements necessary to realize this protocol: a magnetic field generator adapted to a specific imaging setup and the aforementioned superparamagnetic microbeads. Then we detail the different steps of a protocol that can be used on diverse cell types, adherent or not.

Indexed as

Actin CytoskeletonActinsAnimalsHumansMagnetic FieldsMicrospheresActinsActin cortexCell mechanicsDynamic measurementImage analysisMagnetic beads

Identifiers

PMID38709482

DOI10.1038/ncomms8764

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.