A simple and unified protocol to purify all seven Escherichia coli RNA polymerase sigma factors.

Barbara Kędzierska, Aleksandra Stodolna, Katarzyna Bryszkowska, Maciej Dylewski, Katarzyna Potrykus

Abstract read

In one paragraph

Article in Journal of applied genetics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Subtractive Proteomics-Based Prioritization of Drug Targets in Enterococcus faecalis V583 and Structure-Based Identification of Plant-Derived Inhibitors Targeting Alanine Racemase: A High Priority Target.Molecular biotechnology · 2026
Article

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5 · Who and what money

Authors and funding

5 authors.

Barbara KędzierskaDepartment of Bacterial Molecular Genetics, Faculty of Biology, University of Gdańsk, Gdańsk, Poland.

Aleksandra StodolnaDepartment of Bacterial Molecular Genetics, Faculty of Biology, University of Gdańsk, Gdańsk, Poland.

Katarzyna BryszkowskaDepartment of Bacterial Molecular Genetics, Faculty of Biology, University of Gdańsk, Gdańsk, Poland.

Maciej DylewskiDepartment of Bacterial Molecular Genetics, Faculty of Biology, University of Gdańsk, Gdańsk, Poland.

Katarzyna PotrykusDepartment of Bacterial Molecular Genetics, Faculty of Biology, University of Gdańsk, Gdańsk, Poland. katarzyna.potrykus@ug.edu.pl.ORCID http://orcid.org/0000-0002-4902-3348

Funding

Narodowe Centrum Nauki UMO-2021/43/B/NZ2/00855

6 · The paper itself

Abstract

RNA polymerase sigma factors are indispensable in the process of bacterial transcription. They are responsible for a given gene's promoter region recognition on template DNA and hence determine specificity of RNA polymerase and play a significant role in gene expression regulation. Here, we present a simple and unified protocol for purification of all seven Escherichia coli RNA polymerase sigma factors. In our approach, we took advantage of the His

Indexed as

DNA-Directed RNA PolymerasesEscherichia coliEscherichia coli ProteinsSigma FactorGene Expression Regulation, BacterialPromoter Regions, GeneticTranscription, GeneticDNA-Directed RNA PolymerasesEscherichia coli ProteinsSigma FactorProtein purificationRNA polymeraseSigma factorSigma subunitSUMO-tagTranscription

Identifiers

PMID38709457

DOI10.1007/s13353-024-00870-3

PMCPMC11310293

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.