Evidence map›Paper›PMID 38708688›Full record

ArticleSmall methods2024

Generation of a Human Induced Pluripotent Stem Cell Line Expressing a Magnetic Resonance Imaging Reporter Gene.

Yura Son, Pengsheng Li, Dakota Ortega, Huiliang Qiu, Hannah Prachyl, Ming Yang, Wuqiang Zhu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Small methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yura SonDepartment of Cardiovascular Medicine, Department of Physiology and Biomedical Engineering, and Center for Regenerative Biotherapeutics, Mayo Clinic Arizona, Scottsdale, AZ, 85259, USA.ORCID 0000-0002-4069-1243
Pengsheng LiDepartment of Cardiovascular Medicine, Department of Physiology and Biomedical Engineering, and Center for Regenerative Biotherapeutics, Mayo Clinic Arizona, Scottsdale, AZ, 85259, USA.
Dakota OrtegaKE Biosciences Preclinical Imaging Center, Arizona State University, Tempe, AZ, 85281, USA.
Huiliang QiuDepartment of Cardiovascular Medicine, Department of Physiology and Biomedical Engineering, and Center for Regenerative Biotherapeutics, Mayo Clinic Arizona, Scottsdale, AZ, 85259, USA.
Hannah PrachylDepartment of Cardiovascular Medicine, Department of Physiology and Biomedical Engineering, and Center for Regenerative Biotherapeutics, Mayo Clinic Arizona, Scottsdale, AZ, 85259, USA.
Ming YangDepartment of Radiology, Mayo Clinic Arizona, Scottsdale, AZ, 85259, USA.
Wuqiang ZhuDepartment of Cardiovascular Medicine, Department of Physiology and Biomedical Engineering, and Center for Regenerative Biotherapeutics, Mayo Clinic Arizona, Scottsdale, AZ, 85259, USA.ORCID 0000-0001-5217-7789

Funding

Cardiomyocyte Non-autonomous Factors and Cardiac Regeneration in Large MammalsR01HL156855 · NHLBI · MAYO CLINIC ARIZONA · PI Wuqiang Zhu · 2022 to 2026
$3.3M
Strategies to Enhance Engineered Heart Tissue Based Myocardial RepairR01HL162747 · NHLBI · MAYO CLINIC ARIZONA · PI Jingwei Xie, Wuqiang Zhu · 2023 to 2026
$2.9M
Electrically Conductive Stem Cell Derived MicroEngineered Heart Tissue for Regeneration of Injured MyocardiumR01HL172784 · NHLBI · MAYO CLINIC ARIZONA · PI Mehdi Nikkhah, Wuqiang Zhu · 2024 to 2026
$2.0M
Myocardial Repair with a Novel Engineered Cardiac Muscle PatchR01HL142627 · NHLBI · MAYO CLINIC ARIZONA · PI ZHU, WUQIANG · 2019 to 2022
$1.7M
Myocardial Repair with a Novel Engineered Cardiac Muscle PatchR56HL142627 · NHLBI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI ZHU, WUQIANG · 2018 to 2018
$371k
NHLBI NIH HHS HL142627NHLBI NIH HHS HL156855NHLBI NIH HHS HL162747NHLBI NIH HHS R01 HL142627NHLBI NIH HHS R01 HL156855NHLBI NIH HHS R01 HL162747NHLBI NIH HHS R01 HL172784NHLBI NIH HHS R56 HL142627
6 · The paper itself

Abstract

The objective of the current study is to develop a new method for tracking transplanted human induced pluripotent stem cells-derived cardiomyocytes (hiPSC-CMs) using magnetic resonance imaging (MRI). The CRISPR/dCas9 activation system is employed to overexpress ferritin heavy chain (FHC) in hiPSC-CMs. The mRNA and protein expression of FHC in hiPSC and hiPSC-CMs significantly increased after transfection. Iron chloride does not affect the cell viability in a concentration range from 0 to 2000 µm. hiPSCs overexpressing FHC (hiPSC- FHC

Indexed as

Genes, ReporterInduced Pluripotent Stem CellsMagnetic Resonance ImagingMyocytes, CardiacAnimalsCell DifferentiationCell LineCRISPR-Cas SystemsHumansMiceCRISPR/dCas9 activation systemferritin heavy chainhuman‐induced pluripotent stem cells‐derived cardiomyocytesmagnetic resonance imaging

Identifiers

PMID38708688
PMCPMC11483194

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.