Evidence map›Paper›PMID 38706928›Full record

Article3 Biotech2024

Validation of a simplified small-scale DNA extraction protocol from wine by quantitative real-time PCR.

Monica Scali, Giacomo Spinsanti, Rita Vignani

Abstract read
In one paragraph

Article in 3 Biotech, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Monica ScaliDepartment of Life Sciences, University of Siena, 53100 Siena, Italy.ORCID 0000-0002-8551-8823
Giacomo SpinsantiDepartment of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy.
Rita VignaniDepartment of Life Sciences, University of Siena, 53100 Siena, Italy.ORCID 0000-0002-7024-5965

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the present study, we compared a simplified small-scale purification protocol to obtain DNA admixtures out of wine, with our large-scale published method. The extraction methods must provide DNA free of PCR inhibitors, that can interfere with DNA amplification. To evaluate the efficiency of grapevine's nuclear DNA extraction from wine, the new protocol was also compared in terms of purity and yield to the DNA obtained out of grapevine's ( Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-024-03992-x.

Indexed as

Molecular authenticationmyICReal-time PCRVitis viniferaWine DNA

Identifiers

PMID38706928
PMCPMC11065827

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.