ArticleiScience2024
A robust vessel-labeling pipeline with high tissue clearing compatibility for 3D mapping of vascular networks.
Article in iScience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- A dual-modality workflow for quantifying microvascular structure in human temporal lobe epilepsy.IBRO neuroscience reports · 2026Article
- 3D vascular mapping reveals multi-organ injury following severe acute pancreatitis.Theranostics · 2026Article
- Advances in tissue optical clearing for 3D imaging in large animal.Frontiers of optoelectronics · 2025Review
- An Improved SHANEL Procedure for Clearing and Staining Brain Tissue from Multiple Species.International journal of molecular sciences · 2025Article
- Tissue Optical Clearing Imaging from Ex vivo toward In vivo.BME frontiers · 2024Review
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The combination of vessel-labeling, tissue-clearing, and light-sheet imaging techniques provides a potent tool for accurately mapping vascular networks, enabling the assessment of vascular remodeling in vascular-related disorders. However, most vascular labeling methods face challenges such as inadequate labeling efficiency or poor compatibility with current tissue clearing technology, which significantly undermines the image quality. To address this limitation, we introduce a vessel-labeling pipeline, termed Ultralabel, which relies on a specially designed dye hydrogel containing lysine-fixable dextran and gelatins for double enhancement. Ultralabel demonstrates not only excellent vessel-labeling capability but also strong compatibility with all tissue clearing methods tested, which outperforms other vessel-labeling methods. Consequently, Ultralabel enables fine mapping of vascular networks in diverse organs, as well as multi-color labeling alongside other labeling techniques. Ultralabel should provide a robust and user-friendly method for obtaining 3D vascular networks in different biomedical applications.
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Registered trials
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