Evidence map›Paper›PMID 38705992›Full record

ArticleOne health outlook2024

Detection and antibiogram profile of diarrheagenic Escherichia coli isolated from two abattoir settings in northwest Ethiopia: a one health perspective.

Solomon Lulie Abey, Mersha Teka, Abebe Belete Bitew, Wassie Molla, Mebrat Ejo, Gashaw Getaneh Dagnaw, Takele Adugna, Seleshe Nigatu, Bemrew Admassu Mengistu, Mebrie Zemene Kinde and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in One health outlook, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Shiga Toxin-ProducingMicroorganisms · 2025
    Review
  3. Antimicrobial ResistantPathogens (Basel, Switzerland) · 2025
    Review
  4. Review
  5. DiarrheagenicInternational journal of molecular sciences · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Solomon Lulie AbeyDepartment of Veterinary Pathobiology, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, P.O. Box 196, Ethiopia. solomonlulie@gmail.com.ORCID http://orcid.org/0009-0003-9464-9029
Mersha TekaDepartment of Veterinary Pathobiology, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, P.O. Box 196, Ethiopia.
Abebe Belete BitewDepartment of Veterinary Epidemiology and Public Health, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, P.O. Box 196, Ethiopia.ORCID http://orcid.org/0000-0001-5043-1528
Wassie MollaDepartment of Veterinary Epidemiology and Public Health, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, P.O. Box 196, Ethiopia.
Mebrat EjoVaccine and Diagnostics Research & Development Division, Armauer Hansen Research Institute (AHRI), 1005, Addis Ababa, Ethiopia.
Gashaw Getaneh DagnawDepartment of Veterinary Biomedical Sciences, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, P.O. Box 196, Ethiopia.ORCID http://orcid.org/0000-0002-8773-2698
Takele AdugnaDepartment of Veterinary Clinical Medicine, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, P.O. Box 196, Ethiopia.
Seleshe NigatuDepartment of Veterinary Epidemiology and Public Health, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, P.O. Box 196, Ethiopia.
Bemrew Admassu MengistuDepartment of Veterinary Biomedical Sciences, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, P.O. Box 196, Ethiopia.
Mebrie Zemene KindeDepartment of Veterinary Biomedical Sciences, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, P.O. Box 196, Ethiopia.
Adugna BerjuDepartment of Veterinary Epidemiology and Public Health, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, P.O. Box 196, Ethiopia.
Mequanint Addisu BeleteInstitute of Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia.ORCID http://orcid.org/0000-0002-1951-2562
Wudu TemesgenDepartment of Veterinary Epidemiology and Public Health, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, P.O. Box 196, Ethiopia.
Shimelis DagnachewDepartment of Veterinary Pathobiology, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, P.O. Box 196, Ethiopia.
Tesfaye Sisay TesemaInstitute of Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia.ORCID http://orcid.org/0000-0002-4103-5029

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDiarrheagenic Escherichia coli (E. coli) is a zoonotic pathogen that contaminates abattoir workers, slaughter environments, slaughter equipment, and carcasses during abattoir processing. Infection with E. coli is associated with the consumption of contaminated food and water, and it is a potential threat to the health and welfare of both humans and animals. Hence, this study aimed to detect diarrheagenic E. coli and assess its antibiogram profile in two abattoir settings, in one health lens.

methodsA cross-sectional study in one health approach was conducted from December 2020 to June 2021. A total of 384 samples from abattoir workers' hands, carcasses, knives, cattle feces, abattoir water and effluents were collected. Bacterial culture and biochemical tests were conducted to isolate E. coli, while conventional polymerase chain reaction was performed to identify virulence genes. The antibiogram of diarrheagenic E. coli was tested against nine antimicrobials using the Kirby Bauer disk diffusion method.

resultsA total of 115 (29.95%) E. coli were isolated from the 384 samples, and from these isolates, about 17 (14.8%) were confirmed to be diarrheagenic E. coli (DEC). Among the DEC pathotypes, nine (52.94%), five (29.4%), and three (17.65%) were Shiga toxin-producing, enterohemorrhagic, and enterotoxigenic E. coli, respectively. While 14 (82.35%) DEC isolates harbored the stx2 gene, five (29.41%) the eae gene, five (29.41%) the hlyA gene and three (17.65%) harbored the st gene. All the DEC isolates were resistant to erythromycin and vancomycin; whereas, they were susceptible to ampicillin, nalidixic acid and norfloxacin. Furthermore, 64.7% of DEC isolates showed resistance to both ceftazidime and kanamycin and 88.24% of the isolates showed multidrug resistance.

conclusionThis study detected DEC isolates having different virulence genes, which showed single and multiple antimicrobial resistance. Given the existing poor hygienic and sanitary practices along the abattoir-to-table food chain, coupled with the habit of raw meat consumption, this result indicates a potential public and animal health risk from the pathogen and antimicrobial resistance.

Indexed as

Abattoir settingAntibiogram profileCarcassesDiarrheagenic E. ColiVirulence genes

Identifiers

PMID38705992
PMCPMC11071326

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.