Evidence map›Paper›PMID 38705960›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2024

In vitro effects of alginate lyase SG4 + produced by Paenibacillus lautus alone and combined with antibiotics on biofilm formation by mucoid Pseudomonas aeruginosa.

Kriti Kanwar, Deepika Sharma, Harjodh Singh, Mohinder Pal, Rajneesh Bandhu, Wamik Azmi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Kriti Kanwar *Department of Biotechnology, Himachal Pradesh University, Summerhill Shimla, H.P, 171005, India. kanwar30kriti@gmail.com.ORCID http://orcid.org/0000-0002-3774-0024
Deepika Sharma *Chandigarh Group of College, Landran, Kharar- Banur Highway, Sector 112, Greater Mohali, Panjab, 140307, India.
Harjodh SinghChandigarh Group of College, Landran, Kharar- Banur Highway, Sector 112, Greater Mohali, Panjab, 140307, India.
Mohinder PalChandigarh Group of College, Landran, Kharar- Banur Highway, Sector 112, Greater Mohali, Panjab, 140307, India.
Rajneesh BandhuDepartment of Biotechnology, Himachal Pradesh University, Summerhill Shimla, H.P, 171005, India.
Wamik AzmiDepartment of Biotechnology, Himachal Pradesh University, Summerhill Shimla, H.P, 171005, India. wamikazmi@rediffmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Alginate is a major extra polymeric substance in the biofilm formed by mucoid Pseudomonas aeruginosa. It is the main proven perpetrator of lung infections in patients suffering from cystic fibrosis. Alginate lyases are very important in the treatment of cystic fibrosis. This study evaluated the role of standalone and in conjugation, effect of alginate lyase of SG4 + isolated from Paenibacillus lautus in enhancing in vitro bactericidal activity of gentamicin and amikacin on mucoid P. aeruginosa. Using Response Surface Methodology (RSM) alginate lyase SG4 + production was optimized in shake flask and there 8.49-fold enhancement in enzyme production. In fermenter, maximum growth (10.15 mg/ml) and alginate lyase (1.46 International Units) production, 1.71-fold was increased using Central Composite Design (CCD). Further, fermentation time was reduced from 48 to 20 h. To the best of our knowledge this is the first report in which CCD was used for fermenter studies to optimize alginate lyase production. The K

Indexed as

Anti-Bacterial AgentsBiofilmsPaenibacillusPolysaccharide-LyasesPseudomonas aeruginosaAlginatesAmikacinBacterial ProteinsFermentationGentamicinsMicrobial Sensitivity TestsAlginatesAmikacinAnti-Bacterial AgentsBacterial ProteinsGentamicinspoly(beta-D-mannuronate) lyasePolysaccharide-LyasesAlginate lyase SG4 +BiofilmsFermenterRSM

Identifiers

PMID38705960
PMCPMC11153421

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.