Evidence map›Paper›PMID 38704764›Full record

ArticleMolecular biology reports2024

Therapeutic potential of Lactobacillus casei and Chlorella vulgaris in high-fat diet-induced non-alcoholic fatty liver disease (NAFLD)-associated kidney damages: a stereological study.

Haniyeh Keyghobadi, Hadis Bozorgpoursavadjani, Farhad Koohpeyma, Nazanin Mohammadipoor, Marzieh Nemati, Farshad Dehghani, Iman Jamhiri, Gholamhossein Keighobadi, Sanaz Dastghaib

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

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3citing papers in PubMed
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3 · Its place in the literature

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3 citing papers in PubMed.

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5 · Who and what money

Authors and funding

9 authors.

Haniyeh KeyghobadiDepartment of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran.
Hadis BozorgpoursavadjaniDepartment of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran.
Farhad KoohpeymaEndocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Nazanin MohammadipoorNutrition and Integrative Physiology, Florida State University, Tallahassee, FL, USA.
Marzieh NematiEndocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Farshad DehghaniStudent Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
Iman JamhiriMolecular Dermatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Gholamhossein KeighobadiDeparment of Agriculture Management, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
Sanaz DastghaibEndocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. suny.respina@gmail.com.ORCID http://orcid.org/0000-0001-8553-9221

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe non-alcoholic fatty liver disease (NAFLD) is prevalent in as many as 25% of adults who are afflicted with metabolic syndrome. Oxidative stress plays a significant role in the pathophysiology of hepatic and renal injury associated with NAFLD. Therefore, probiotics such as Lactobacillus casei (LBC) and the microalga Chlorella vulgaris (CV) may be beneficial in alleviating kidney injury related to NAFLD. MATERIALS AND

methodsThis animal study utilized 30 C57BL/6 mice, which were evenly distributed into five groups: the control group, the NAFLD group, the NAFLD + CV group, the NAFLD + LBC group, and the NAFLD + CV + LBC group. A high-fat diet (HFD) was administered to induce NAFLD for six weeks. The treatments with CV and LBC were continued for an additional 35 days. Biochemical parameters, total antioxidant capacity (TAC), and the expression of kidney damage marker genes (KIM 1 and NGAL) in serum and kidney tissue were determined, respectively. A stereological analysis was conducted to observe the structural changes in kidney tissues.

resultsA liver histopathological examination confirmed the successful induction of NAFLD. Biochemical investigations revealed that the NAFLD group exhibited increased ALT and AST levels, significantly reduced in the therapy groups (p < 0.001). The gene expression levels of KIM-1 and NGAL were elevated in NAFLD but were significantly reduced by CV and LBC therapies (p < 0.001). Stereological examinations revealed reduced kidney size, volume, and tissue composition in the NAFLD group, with significant improvements observed in the treated groups (p < 0.001).

conclusionThis study highlights the potential therapeutic efficacy of C. vulgaris and L. casei in mitigating kidney damage caused by NAFLD. These findings provide valuable insights for developing novel treatment approaches for managing NAFLD and its associated complications.

Indexed as

Chlorella vulgarisDiet, High-FatKidneyLacticaseibacillus caseiMice, Inbred C57BLNon-alcoholic Fatty Liver DiseaseProbioticsAnimalsAntioxidantsDisease Models, AnimalKidney DiseasesLiverMaleMiceOxidative StressAntioxidantsHigh fat dietKIM-1NGALNon-alcoholic fatty liver diseaseOxidative stress

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PMID38704764

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