Evidence map›Paper›PMID 38704038›Full record

ArticleJournal of microbiological methods2024

Revisiting gas-chromatography/mass-spectrometry molar response factors for quantitative analysis (FID or TIC) of glycosidic linkages in polysaccharides produced by oral bacterial biofilms.

Alexander J Kendall, Polliana M C Scaffa, Matthew G Logan, Carmem S Pfeifer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of microbiological methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Alexander J KendallOregon Health & Science University, School of Dentistry, Division of Biomaterial and Biomedical Sciences, Portland, OR, USA.
Polliana M C ScaffaOregon Health & Science University, School of Dentistry, Division of Biomaterial and Biomedical Sciences, Portland, OR, USA.
Matthew G LoganOregon Health & Science University, School of Dentistry, Division of Biomaterial and Biomedical Sciences, Portland, OR, USA.
Carmem S PfeiferOregon Health & Science University, School of Dentistry, Division of Biomaterial and Biomedical Sciences, Portland, OR, USA. Electronic address: pfeiferc@ohsu.edu.

Funding

Novel polymeric materials with improved durability in the oral environment: tailoring responses to host and bacterial enzymes with anti-proteolytic and ecology-based antimicrobial approaches.R35DE029083 · NIDCR · OREGON HEALTH & SCIENCE UNIVERSITY · PI Carmem S. Pfeifer · 2019 to 2026
$8.1M
Host and bacterial enzyme-mediated approaches to produce durable direct and indirect restorationsR01DE028757 · NIDCR · OREGON HEALTH & SCIENCE UNIVERSITY · PI PFEIFER, CARMEM S. · 2019 to 2019
$495k
NIDCR NIH HHS R01 DE028757NIDCR NIH HHS R35 DE029083
6 · The paper itself

Abstract

Methylation analysis was performed on methylated alditol acetate standards and Streptococcus mutans extracellular polymeric substances (EPS) produced from wild-type and Gtf knockout strains (∆GtfB, ∆GtfB, and ∆GtfD). The methylated alditol acetate standards were representative of glycosidic linkages found in S. mutans EPS and were used to calibrate the GC-MS system for an FID detector and MS (TIC) and produce molar response factor, a necessary step in quantitative analysis. FID response factors were consistent with literature values (Sweet et al., 1975) and found to be the superior option for quantitative results, although the TIC response factors now give researchers without access to an FID detector a needed option for molar response factor correction. The GC-MS analysis is then used to deliver the ratio of the linkage types within a biofilm.

Indexed as

BiofilmsGas Chromatography-Mass SpectrometryPolysaccharides, BacterialStreptococcus mutansExtracellular Polymeric Substance MatrixGlycosidesMethylationPolysaccharidesGlycosidesPolysaccharidesPolysaccharides, BacterialExtra-cellular polymeric substanceGlucansMolar response factorOral biofilms

Identifiers

PMID38704038
PMCPMC11102815

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.