Isolation of Human Progenitor Epidermal Cells on Collagen Type IV and Analysis of Their Markers with Flow Cytometry and PCR Methods.

Milena Deptuła, Małgorzata Zawrzykraj, Paulina Słonimska, Michał Pikuła

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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

4 authors.

Milena DeptułaLaboratory of Tissue Engineering and Regenerative Medicine, Division of Embryology, Medical University of Gdansk, Gdansk, Poland. milenadeptula@gumed.edu.pl.

Małgorzata ZawrzykrajDivision of Clinical Anatomy, Medical University of Gdansk, Gdansk, Poland.

Paulina SłonimskaLaboratory for Regenerative Biotechnology, Gdansk University of Technology, Gdansk, Poland.

Michał PikułaLaboratory of Tissue Engineering and Regenerative Medicine, Division of Embryology, Medical University of Gdansk, Gdansk, Poland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Epidermal stem cells, located in the skin, together with keratinocytes are transplanted in regenerative therapies, e.g., for the treatment of burns or other wounds. Here, we describe the protocol of their enzymatic isolation from human skin. It includes separation of the epidermis form the dermis by incubation with dispase followed by cell isolation for epidermis by digestion with trypsin. Cell isolated with this method can be seeded on collagen IV-coated dishes. The methods of analysis of epidermal stem cells markers (e.g., CD71, CD29) with flow cytometry and RT-PCR are also included.

Indexed as

BiomarkersCell SeparationCollagen Type IVEpidermal CellsFlow CytometryStem CellsAntigens, CDEndopeptidasesHumansPolymerase Chain ReactionAntigens, CDBiomarkersCollagen Type IVdispaseEndopeptidasesCell characterizationCell cultureCell isolationEpidermal stem cellsEpidermisFlow cytometryKeratinocytesRT-PCRSkin

Identifiers

PMID38700832

DOI10.1016/j.advms.2019.03.001

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.