Evidence map›Paper›PMID 38698909›Full record

ArticlemicroPublication biology2024

Analysis of antibodies from whole-cell immunization by a tANCHOR cell-based ELISA.

Hubert Bernauer, Anja Schlör, Josef Maier, Katja Hanack, Norbert Bannert, Daniel Ivanusic

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in microPublication biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hubert BernauerATG:biosynthetics GmbH, Weberstraße 40, 79249 Merzhausen, Germany.
Anja Schlörnew/era/mabs GmbH, August-Bebel-Str. 89, 14482 Potsdam, Germany.
Josef MaierATG:biosynthetics GmbH, Weberstraße 40, 79249 Merzhausen, Germany.
Katja Hanacknew/era/mabs GmbH, August-Bebel-Str. 89, 14482 Potsdam, Germany.
Norbert BannertSexually transmitted bacterial pathogens and HIV (FG18), Robert Koch-Institute, Nordufer 20, 13353 Berlin, Germany.
Daniel IvanusicSexually transmitted bacterial pathogens and HIV (FG18), Robert Koch-Institute, Nordufer 20, 13353 Berlin, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Monitoring specific antibodies derived from whole-cell immunization through cell-based ELISA methods poses challenges due to humoral responses against various cell proteins. In this report, we outline a technique involving pre-adsorption on cells to remove undesirable antibodies from immune serum. This step provides the subsequent monitoring of antibodies specific to the targeted antigen using a tANCHOR-based ELISA. Notably, this approach accelerates result acquisition, eliminating the necessity to purify the expressed antigen or obtain a customized peptide for coating assay plates.

Identifiers

PMID38698909
PMCPMC11063790

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.