Evidence map›Paper›PMID 38696722›Full record

ArticleProtein engineering, design & selection : PEDS2024

Modular and integrative activity reporters enhance biochemical studies in the yeast ER.

Samantha G Martinusen, Ethan W Slaton, Sage E Nelson, Marian A Pulgar, Julia T Besu, Cassidy F Simas, Carl A Denard

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein engineering, design & selection : PEDS, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Samantha G MartinusenDepartment of Chemical Engineering, University of Florida, Gainesville, 32611, USA.
Ethan W SlatonDepartment of Chemical Engineering, University of Florida, Gainesville, 32611, USA.
Sage E NelsonDepartment of Chemical Engineering, University of Florida, Gainesville, 32611, USA.
Marian A PulgarDepartment of Chemical Engineering, University of Florida, Gainesville, 32611, USA.
Julia T BesuDepartment of Biology, University of Florida, Gainesville, 32611, USA.
Cassidy F SimasJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, 32611, USA.
Carl A DenardDepartment of Chemical Engineering, University of Florida, Gainesville, 32611, USA.ORCID 0000-0002-2804-9426

Funding

Reprogramming proteases: tackling human diseases with next-generation modulatorsR35GM146821 · NIGMS · UNIVERSITY OF FLORIDA · PI Carl Denard · 2022 to 2026
$1.6M
Machine Learning-Guided Engineering of Protease ModulatorsR21GM144812 · NIGMS · UNIVERSITY OF FLORIDA · PI DENARD, CARL · 2022 to 2022
$219k
NIGMS NIH HHS R21 GM144812NIGMS NIH HHS R35 GM146821
6 · The paper itself

Abstract

The yeast endoplasmic reticulum sequestration and screening (YESS) system is a broadly applicable platform to perform high-throughput biochemical studies of post-translational modification enzymes (PTM-enzymes). This system enables researchers to profile and engineer the activity and substrate specificity of PTM-enzymes and to discover inhibitor-resistant enzyme mutants. In this study, we expand the capabilities of YESS by transferring its functional components to integrative plasmids. The YESS integrative system yields uniform protein expression and protease activities in various configurations, allows one to integrate activity reporters at two independent loci and to split the system between integrative and centromeric plasmids. We characterize these integrative reporters with two viral proteases, Tobacco etch virus (TEVp) and 3-chymotrypsin like protease (3CLpro), in terms of coefficient of variance, signal-to-noise ratio and fold-activation. Overall, we provide a framework for chromosomal-based studies that is modular, enabling rigorous high-throughput assays of PTM-enzymes in yeast.

Indexed as

Endoplasmic ReticulumSaccharomyces cerevisiaeEndopeptidasesGenes, ReporterPlasmidsProtein Processing, Post-TranslationalEndopeptidasesenzyme activity reportershigh-throughput screening and selectionProtease engineeringyeast endoplasmic reticulum sequestration and screeningyeast surface display

Identifiers

PMID38696722
PMCPMC11091476

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.