Evidence map›Paper›PMID 38696094›Full record

ArticleProbiotics and antimicrobial proteins2025

Different Detection Strategies of Pediocin-Like Produced by Pediococcus pentosaceus.

Hongbiao Li, Huojian Zheng, Lanxin Li, Yi Lin, Qiuxiang Cheng, Liping Wang, Yueliang Zhao, Zhenmin Liu

Abstract read
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Article in Probiotics and antimicrobial proteins, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 94% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Enhancement of Pediocin Production inFoods (Basel, Switzerland) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Hongbiao LiCollege of Food Science and Technology, Shanghai Ocean University, Nanhui New City, No.999, Huchenghuan Rd, Shanghai, 201306, People's Republic of China.
Huojian Zheng *College of Food Science and Technology, Shanghai Ocean University, Nanhui New City, No.999, Huchenghuan Rd, Shanghai, 201306, People's Republic of China.
Lanxin LiCollege of Food Science and Technology, Shanghai Ocean University, Nanhui New City, No.999, Huchenghuan Rd, Shanghai, 201306, People's Republic of China.
Yi Lin *College of Food Science and Technology, Shanghai Ocean University, Nanhui New City, No.999, Huchenghuan Rd, Shanghai, 201306, People's Republic of China.
Qiuxiang ChengState Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China.
Liping WangCollege of Food Science and Technology, Shanghai Ocean University, Nanhui New City, No.999, Huchenghuan Rd, Shanghai, 201306, People's Republic of China. lpwang@shou.edu.cn.
Yueliang ZhaoCollege of Food Science and Technology, Shanghai Ocean University, Nanhui New City, No.999, Huchenghuan Rd, Shanghai, 201306, People's Republic of China.
Zhenmin LiuState Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai, 200436, People's Republic of China. liuzhenmin@brightdairy.com.
Shanghai Ocean University · CNShanghai Jiao Tong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, Pediococcus pentosaceus C-2-1 and C23221 contained genes encoding penocin and pediocin PA-1, mined by antiSMASH. The penocin structural gene pedA from Pediococcus pentosaceus C-2-1 was successfully expressed in Escherichia coli BL21. The presence of a 6.5 kDa recombinant penocin was confirmed by Tricine-SDS-PAGE, and the specific activity increased by 1.54-fold. The bacteriocins produced by Pediococcus pentosaceus C23221 were purified using acetic ether extraction, Sepharose Fast Flow, Sephadex G-25 gel chromatography, and reversed-phase high-performance liquid chromatography (RP-HPLC); the amino acid sequence of this bacteriocin was identical to pediocin PA-1 by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), which confirmed the expression of pediocin PA-1 gene; and the specific activity increased by 24.39-fold. The heterologous expression and purification of bacteriocins have proved the expression of pediocin-like produced by Pediococcus pentosaceus. This provides a theoretical basis for the subsequent development and application of pediocin-like.

Indexed as

BacteriocinsPediocinsPediococcus pentosaceusAmino Acid SequenceChromatography, High Pressure LiquidEscherichia coliTandem Mass SpectrometryBacteriocinspediocin PA-1PediocinsHeterologous expressionPediocin PA-1PenocinPurification

Identifiers

PMID38696094
OpenAlexW4396581471

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.