ArticleBMC genomics2024
DengueSeq: a pan-serotype whole genome amplicon sequencing protocol for dengue virus.
Article in BMC genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 30 citations in OpenAlex.
- Article
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- A highly sensitive amplicon sequencing workflow for genomic surveillance of Usutu virus.Virology journal · 2026Article
- Fatal European subtype tick-borne encephalitis in a fully vaccinated immunocompetent child: a case report with viral sequencing.BMC infectious diseases · 2026Article
- Frequent introductions and climate suitability drive increasing dengue risk in Florida.medRxiv : the preprint server for health sciences · 2026Article
- Evolutionary history of Jamestown Canyon virus reveals complex multi-vector ecology.Current biology : CB · 2026Article
- A tiled amplicon protocol for culture-free whole-genome sequencing ofJournal of clinical microbiology · 2026Article
- Evolutionary history of Jamestown Canyon virus disentangles complex multi-vector ecology.bioRxiv : the preprint server for biology · 2026Article
- Genomic characterization of a severe West Nile Virus transmission season using a single reaction amplicon sequencing approach.PLoS neglected tropical diseases · 2026Article
- Phylogenetic insights into the transmission dynamics of arthropod-borne viruses.Nature reviews. Genetics · 2026Review
- Epidemiology and severity risk factors of dengue virus infection during the 2023-2024 outbreak in Colombia.PLoS neglected tropical diseases · 2025Article
- Genomic epidemiology of dengue virus 2 and 3 reveals repeated introductions and exportations of several lineages in Colombia.medRxiv : the preprint server for health sciences · 2025Article
- Viral Metagenomic Next-Generation Sequencing for One Health Discovery and Surveillance of (Re)Emerging Viruses: A Deep Review.International journal of molecular sciences · 2025Review
- Evidence of dengue virus transmission and a diversebioRxiv : the preprint server for biology · 2025Article
- Universal amplification and sequencing of foot-and-mouth disease virus complete genomes using nanopore technology.BMC genomics · 2025Article
- CDC dengue typing kit fails to detect dengue virus 2 sylvatic genotype.Journal of clinical microbiology · 2025Article
- Complete genome sequence of dengue virus serotype 2 obtained from Chattogram, Bangladesh.Microbiology resource announcements · 2025Article
- Whole genome sequencing and phylogenetic analysis of dengue virus in Central Nepal from 2022 to 2023.BMC global and public health · 2025Article
- Dengue Outbreak Caused by Multiple Virus Serotypes and Lineages, Colombia, 2023-2024.Emerging infectious diseases · 2024Article
- A new lineage nomenclature to aid genomic surveillance of dengue virus.PLoS biology · 2024Article
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Authors and funding
31 authors at 6 institutions in 3 countries.
Funding
Abstract
backgroundThe increasing burden of dengue virus on public health due to more explosive and frequent outbreaks highlights the need for improved surveillance and control. Genomic surveillance of dengue virus not only provides important insights into the emergence and spread of genetically diverse serotypes and genotypes, but it is also critical to monitor the effectiveness of newly implemented control strategies. Here, we present DengueSeq, an amplicon sequencing protocol, which enables whole-genome sequencing of all four dengue virus serotypes.
resultsWe developed primer schemes for the four dengue virus serotypes, which can be combined into a pan-serotype approach. We validated both approaches using genetically diverse virus stocks and clinical specimens that contained a range of virus copies. High genome coverage (>95%) was achieved for all genotypes, except DENV2 (genotype VI) and DENV 4 (genotype IV) sylvatics, with similar performance of the serotype-specific and pan-serotype approaches. The limit of detection to reach 70% coverage was 10-100 RNA copies/μL for all four serotypes, which is similar to other commonly used primer schemes. DengueSeq facilitates the sequencing of samples without known serotypes, allows the detection of multiple serotypes in the same sample, and can be used with a variety of library prep kits and sequencing instruments.
conclusionsDengueSeq was systematically evaluated with virus stocks and clinical specimens spanning the genetic diversity within each of the four dengue virus serotypes. The primer schemes can be plugged into existing amplicon sequencing workflows to facilitate the global need for expanded dengue virus genomic surveillance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.