Evidence map›Paper›PMID 38692961›Full record

ArticleInternational dental journal2024

Biocompatibility and Cytotoxicity of Pulp-Capping Materials on DPSCs, With Marker mRNA Expressions.

Banu Çiçek Tez, Bahar Başak Kızıltan Eliaçık, Pakize Neslihan Taşlı, Hazal Yılmaz, Fikrettin Şahin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International dental journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.1field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Banu Çiçek TezDepartment of Pediatric Dentistry, Faculty of Dentistry, Ankara Medipol University, Ankara, Türkiye.
Bahar Başak Kızıltan EliaçıkDepartment of Pediatric Dentistry, Faculty of Dentistry, University of Health Sciences, Istanbul, Türkiye.
Pakize Neslihan TaşlıDepartment of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul, Türkiye.
Hazal YılmazDepartment of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul, Türkiye.
Fikrettin ŞahinDepartment of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul, Türkiye.
Yeditepe University · TRAnkara Medipol ÜniversitesiUniversity of Health Sciences Antigua · AG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesThe present study aimed to (1) investigate biocompatibility and cytotoxicity of pulp-capping materials on viability of human dental pulp stem cells (hDPSCs); (2) determine angiogenic, odontogenic, and osteogenic marker mRNA expressions; and (3) observe changes in surface morphology of the hDPSCs using scanning electron microscopy (SEM).

methodsImpacted third molars were used to isolate the hDPSCs, which were treated with extract-release fluids of the pulp-capping materials (Harvard BioCal-Cap, NeoPUTTY MTA, TheraCal LC, and Dycal). Effects of the capping materials on cell viability were assessed using 3-(4,5-di-methyl-thiazol-2-yl)-5-(3-carboxy-methoxy-phenyl)-2-(4-sulfo-phenyl)-2H-tetrazolium (MTS) assay and the apoptotic/necrotic cell ratios and reactive oxygen species (ROS) levels from flow cytometry. Marker expressions (alkaline phosphatase [ALP], osteocalcin [OCN], collagen type I alpha 1 [Col1A], secreted protein acidic and rich in cysteine [SPARC], osteonectin [ON], and vascular endothelial growth factor [VEGF]) were determined by quantitative reverse-transcription polymerase chain reaction. Changes in surface morphology of the hDPSCs were visualised by SEM.

resultsThe MTS assay results at days 1, 3, 5, and 7 indicated that Harvard BioCal-Cap, NeoPUTTY MTA, and TheraCal LC did not adversely affect cell viability when compared with the control group. According to the MTS assay results at day 14, no significant difference was found amongst Dycal, Harvard BioCal-Cap, NeoPUTTY MTA, and TheraCal LC affecting cell viability. Dycal was the only capping material that increased ROS level. High levels of VEGF expression were observed with Harvard BioCal-Cap, TheraCal LC, and NeoPUTTY MTA. NeoPUTTY MTA, and Dycal upregulated OCN expression, whereas TheraCal LC upregulated Col1A and SPARC expression. Only Dycal increased ALP expression. HDSCs were visualized in characteristic spindle morphology on SEM when treated with TheraCal LC and Harvard BioCal-Cap.

conclusionsNeoPUTTY MTA and Harvard BioCal-Cap showed suitable biocompatibility values; in particular, these pulp-capping materials were observed to support the angiogenic marker.

Indexed as

Calcium CompoundsCell SurvivalDental PulpOsteocalcinOsteonectinOxidesPulp Capping and Pulpectomy AgentsReactive Oxygen SpeciesSilicatesVascular Endothelial Growth Factor AAdolescentAlkaline PhosphataseAluminum CompoundsBiocompatible MaterialsBiomarkersCells, CulturedAlkaline PhosphataseAluminum CompoundsBiocompatible MaterialsBiomarkersCalcium CompoundsCollagen Type ICollagen Type I, alpha 1 ChainDrug Combinationsmineral trioxide aggregateOsteocalcinOsteonectinOxidesPulp Capping and Pulpectomy AgentsReactive Oxygen SpeciesRNA, MessengerSilicatesVascular Endothelial Growth Factor AVEGFA protein, humanBiocompatibilityCapping materialsCytotoxicityDental pulp stem cellsmRNA expression

Identifiers

PMID38692961
PMCPMC11561506
OpenAlexW4396512409

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.