Evidence map›Paper›PMID 38689658›Full record

ArticleBioactive materials2024

MMP13-targeted siRNA-loaded micelles for diagnosis and treatment of posttraumatic osteoarthritis.

Dongyang Zhou, Yan Wei, Shihao Sheng, Miaomiao Wang, Jiajing Lv, Bowen Zhao, Xiao Chen, Ke Xu, Long Bai, Yan Wu and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioactive materials, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
15.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 32 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 1 country.

Dongyang ZhouInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China.
Yan WeiInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China.
Shihao ShengDepartment of Orthopedic, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.
Miaomiao WangInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China.
Jiajing LvInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China.
Bowen ZhaoInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China.
Xiao ChenDepartment of Orthopedic, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.
Ke XuInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China.
Long BaiInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China.
Yan WuInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China.
Peiran SongInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China.
Liehu CaoDepartment of Orthopedics, Shanghai Baoshan Luodian Hospital, Baoshan District, Shanghai, 201908, China.
Fengjin ZhouDepartment of Orthopaedics, Honghui Hospital, Xi'an Jiao Tong University, Xi'an, 710000, China.
Hao ZhangDepartment of Orthopedic, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.
Zhongmin ShiDepartment of Orthopedics, Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, 200233, China.
Jiacan SuInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China.
Shanghai University · CNShanghai Jiao Tong University · CNBaoshan College · CNXi'an Honghui Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Posttraumatic osteoarthritis (PTOA) patients are often diagnosed by X-ray imaging at a middle-late stage when drug interventions are less effective. Early PTOA is characterized by overexpressed matrix metalloprotease 13 (MMP13). Herein, we constructed an integrated diagnosis and treatment micelle modified with MMP13 enzyme-detachable, cyanine 5 (Cy5)-containing PEG, black hole quencher-3 (BHQ3), and cRGD ligands and loaded with siRNA silencing MMP13 (siM13), namely ERMs@siM13. ERMs@siM13 could be cleaved by MMP13 in the diseased cartilage tissues to detach the PEG shell, causing cRGD exposure. Accordingly, the ligand exposure promoted micelle uptake by the diseased chondrocytes by binding to cell surface αvβ3 integrin, increasing intracellular siM13 delivery for on-demand MMP13 downregulation. Meanwhile, the Cy5 fluorescence was restored by detaching from the BHQ3-containing micelle, precisely reflecting the diseased cartilage state. In particular, the intensity of Cy5 fluorescence generated by ERMs@siM13 that hinged on the MMP13 levels could reflect the PTOA severity, enabling the physicians to adjust the therapeutic regimen. Finally, in the murine PTOA model, ERMs@siM13 could diagnose the early-stage PTOA, perform timely interventions, and monitor the OA progression level during treatment through a real-time detection of MMP13. Therefore, ERMs@siM13 represents an appealing approach for early-stage PTOA theranostics.

Indexed as

Early-stage posttraumatic osteoarthritis (PTOA)Fluorescence imagingMatrix metalloprotease 13 (MMP 13)MicellessiRNA delivery

Identifiers

PMID38689658
PMCPMC11059470
OpenAlexW4395030345

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.