Evidence map›Paper›PMID 38689209›Full record

ArticleBMC plant biology2024

Modified fructan accumulation through overexpression of wheat fructan biosynthesis pathway fusion genes Ta1SST:Ta6SFT.

Tong Chen, Matthew Hayes, Zhiqian Liu, Daniel Isenegger, John Mason, German Spangenberg

Abstract read
In one paragraph

Article in BMC plant biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Tong ChenAgriculture Victoria, Agribio, Bundoora, VIC, Australia.
Matthew HayesAgriculture Victoria, Agribio, Bundoora, VIC, Australia.
Zhiqian LiuAgriculture Victoria, Agribio, Bundoora, VIC, Australia.
Daniel IseneggerAgriculture Victoria, Agribio, Bundoora, VIC, Australia. Daniel.Isenegger@agriculture.vic.gov.au.
John MasonAgriculture Victoria, Agribio, Bundoora, VIC, Australia.
German SpangenbergAgriculture Victoria, Agribio, Bundoora, VIC, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundFructans are water-soluble carbohydrates that accumulate in wheat and are thought to contribute to a pool of stored carbon reserves used in grain filling and tolerance to abiotic stress.

resultsIn this study, transgenic wheat plants were engineered to overexpress a fusion of two fructan biosynthesis pathway genes, wheat sucrose: sucrose 1-fructosyltransferase (Ta1SST) and wheat sucrose: fructan 6-fructosyltransferase (Ta6SFT), regulated by a wheat ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (TaRbcS) gene promoter. We have shown that T4 generation transgene-homozygous single-copy events accumulated more fructan polymers in leaf, stem and grain when compared in the same tissues from transgene null lines. Under water-deficit (WD) conditions, transgenic wheat plants showed an increased accumulation of fructan polymers with a high degree of polymerisation (DP) when compared to non-transgenic plants. In wheat grain of a transgenic event, increased deposition of particular fructan polymers such as, DP4 was observed.

conclusionsThis study demonstrated that the tissue-regulated expression of a gene fusion between Ta1SST and Ta6SFT resulted in modified fructan accumulation in transgenic wheat plants and was influenced by water-deficit stress conditions.

Indexed as

Bacterial ProteinsFructansHexosyltransferasesPlants, Genetically ModifiedTriticumGene Expression Regulation, PlantGene FusionPlant ProteinsBacterial ProteinsFructansHexosyltransferasesinulosucrasePlant Proteinssucrose - fructan 6-fructosyltransferaseFructanTa1SSTTa6SFTWater deficitWheat

Identifiers

PMID38689209
PMCPMC11059666

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.