Evidence map›Paper›PMID 38688558›Full record

ArticleRNA (New York, N.Y.)2024

Selected humanization of yeast U1 snRNP leads to global suppression of pre-mRNA splicing and mitochondrial dysfunction in the budding yeast.

Subbaiah Chalivendra, Shasha Shi, Xueni Li, Zhiling Kuang, Joseph Giovinazzo, Lingdi Zhang, John Rossi, Jingxin Wang, Anthony J Saviola, Robb Welty and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RNA (New York, N.Y.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Functional Analysis of the Zinc Finger Modules of thebioRxiv : the preprint server for biology · 2024
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors.

Subbaiah Chalivendra *Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA.
Shasha Shi *Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA.
Xueni LiDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA.
Zhiling KuangDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA.
Joseph GiovinazzoDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA.
Lingdi ZhangDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA.
John RossiDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA.
Jingxin WangDepartment of Medicinal Chemistry, University of Kansas, Lawrence, Kansas 66047, USA.
Anthony J SaviolaDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA.ORCID 0000-0001-6890-512X
Robb WeltyDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA.
Shiheng LiuDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California 90095, USA.
Katherine F VaethDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA.
Z Hong ZhouDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California 90095, USA.ORCID 0000-0002-8373-4717
Kirk C HansenDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA.
J Matthew TaliaferroDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA.ORCID 0000-0001-7580-1433
Rui ZhaoDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA rui.zhao@cuanschutz.edu subbaiah.chalivendra@cuanschutz.edu.

Funding

University of Colorado Cancer Center Support Grant - Lung Cancer Patient-Derived Xenografts with Autologous Human Immune SystemsP30CA046934 · NCI · UNIVERSITY OF COLORADO DENVER · PI James V Degregori · 1988 to 2026
$117.0M
Pacific Northwest Center for Cryo-EM - Screening supplementU24GM129547 · NIGMS · OREGON HEALTH & SCIENCE UNIVERSITY · PI EVANS, JAMES E, GOUAUX, JAMES E · 2018 to 2023
$54.8M
Resource for Biocomputing Visualization and InformaticsP41GM103311 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIN, THOMAS E · 2012 to 2017
$8.2M
ChimeraX -- Next Generation Visualization and Analysis Software for Multiscale ModelingR01GM129325 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIN, THOMAS E · 2018 to 2025
$5.2M
High-Resolution CryoEM Reconstruction of Large ComplexesR01GM071940 · NIGMS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI ZHOU, Z HONG · 2006 to 2025
$5.0M
The molecular mechanism of pre-mRNA splicingR35GM145289 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI RUI ZHAO · 2022 to 2026
$4.3M
Training in Translational Research of Lung, Head and Neck CancerT32CA174648 · NCI · UNIVERSITY OF COLORADO DENVER · PI KARAM, SANA D · 2013 to 2022
$4.2M
Understanding the regulatory language of RNA localizationR35GM133385 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI Jefferson Matthew Taliaferro · 2019 to 2026
$3.4M
Modulating gene expression by RNA-targeting chimerasR35GM147498 · NIGMS · UNIVERSITY OF KANSAS LAWRENCE · PI Jingxin Wang · 2022 to 2026
$2.1M
Understanding the structure and function of U1 snRNPR01GM126157 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI ZHAO, RUI · 2018 to 2021
$1.8M
NCI NIH HHS P30 CA046934NCI NIH HHS T32 CA174648NIGMS NIH HHS P41 GM103311NIGMS NIH HHS R01 GM071940NIGMS NIH HHS R01 GM126157NIGMS NIH HHS R01 GM129325NIGMS NIH HHS R35 GM133385NIGMS NIH HHS R35 GM145289NIGMS NIH HHS R35 GM147498NIGMS NIH HHS U24 GM129547
6 · The paper itself

Abstract

The recognition of the 5' splice site (5' ss) is one of the earliest steps of pre-mRNA splicing. To better understand, the mechanism and regulation of 5' ss recognition, we selectively humanized components of the yeast U1 (yU1) snRNP to reveal the function of these components in 5' ss recognition and splicing. We targeted U1C and Luc7, two proteins that interact with and stabilize the yU1 snRNA and the 5' ss RNA duplex. We replaced the zinc-finger (ZnF) domain of yeast U1C (yU1C) with its human counterpart, which resulted in a cold-sensitive growth phenotype and moderate splicing defects. We next added an auxin-inducible degron to yeast Luc7 (yLuc7) protein (to mimic the lack of Luc7Ls in human U1 snRNP). We found that Luc7-depleted yU1 snRNP resulted in the concomitant loss of Prp40 and Snu71 (two other essential yU1 snRNP proteins), and further biochemical analyses suggest a model of how these three proteins interact with each other in the U1 snRNP. The loss of these proteins resulted in a significant growth retardation accompanied by a global suppression of pre-mRNA splicing. The splicing suppression led to mitochondrial dysfunction as revealed by a release of Fe

Indexed as

MitochondriaRibonucleoprotein, U1 Small NuclearRNA PrecursorsRNA SplicingSaccharomyces cerevisiaeHumansRNA Splice SitesSaccharomyces cerevisiae ProteinsSaccharomycetalesRibonucleoprotein, U1 Small NuclearRNA PrecursorsRNA Splice SitesSaccharomyces cerevisiae Proteins5′ splice site recognitionLuc7U1CU1 snRNP

Identifiers

PMID38688558
PMCPMC11251525

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.