Evidence map›Paper›PMID 38687789›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2024

Tailored UPRE2 variants for dynamic gene regulation in yeast.

Chufan Xiao, Xiufang Liu, Yuyang Pan, Yanling Li, Ling Qin, Zhibo Yan, Yunzi Feng, Mouming Zhao, Mingtao Huang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Recombinant Protein Drugs: A 2025 Update.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2026
    Review
  2. Article
  3. Inheritance of extraordinary metabolic activity from parental bacteria individuals.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Chufan Xiao *School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Xiufang Liu *School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Yuyang PanSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Yanling LiSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Ling QinSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Zhibo YanSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Yunzi FengSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Mouming ZhaoSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Mingtao HuangSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.ORCID 0000-0003-2359-1010

Funding

MOE | Fundamental Research Funds for the Central Universities (Fundamental Research Fund for the Central Universities) 2022ZYGXZR102MOST | National Key Research and Development Program of China (NKPs) 2021YFC2100500MOST | National Natural Science Foundation of China (NSFC) 31901013
6 · The paper itself

Abstract

Genetic elements are foundational in synthetic biology serving as vital building blocks. They enable programming host cells for efficient production of valuable chemicals and recombinant proteins. The unfolded protein response (UPR) is a stress pathway in which the transcription factor Hac1 interacts with the upstream unfolded protein response element (UPRE) of the promoter to restore endoplasmic reticulum (ER) homeostasis. Here, we created a UPRE2 mutant (UPRE2m) library. Several rounds of screening identified many elements with enhanced responsiveness and a wider dynamic range. The most active element m84 displayed a response activity 3.72 times higher than the native UPRE2. These potent elements are versatile and compatible with various promoters. Overexpression of

Indexed as

Basic-Leucine Zipper Transcription FactorsGene Expression Regulation, FungalSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsUnfolded Protein ResponseEndoplasmic ReticulumPromoter Regions, GeneticRepressor ProteinsSignal TransductionBasic-Leucine Zipper Transcription FactorsHAC1 protein, S cerevisiaeRepressor ProteinsSaccharomyces cerevisiae ProteinsHac1responsive promoterunfolded protein responseUPRE2 variants

Identifiers

PMID38687789
PMCPMC11087760

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.