Evidence map›Paper›PMID 38687063›Full record

ArticleMicrobiology spectrum2024

Advancing metagenome-assembled genome-based pathogen identification: unraveling the power of long-read assembly algorithms in Oxford Nanopore sequencing.

Zhao Chen, Christopher J Grim, Padmini Ramachandran, Jianghong Meng

Abstract read
In one paragraph

Article in Microbiology spectrum, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Zhao ChenJoint Institute for Food Safety and Applied Nutrition, Center for Food Safety and Security Systems, University of Maryland, College Park, Maryland, USA.ORCID 0000-0002-1784-167X
Christopher J GrimCenter for Food Safety and Applied Nutrition, United States Food and Drug Administration, College Park, Maryland, USA.
Padmini RamachandranCenter for Food Safety and Applied Nutrition, United States Food and Drug Administration, College Park, Maryland, USA.ORCID 0000-0002-3958-3843
Jianghong MengJoint Institute for Food Safety and Applied Nutrition, Center for Food Safety and Security Systems, University of Maryland, College Park, Maryland, USA.

Funding

Cooperative Agreement to Support the Joint Institute for Food Safety and Applied Nutrition (JIFSAN)U01FD001418 · FDA · UNIV OF MARYLAND, COLLEGE PARK · PI Jianghong Meng · 2005 to 2026
$55.4M
FDA HHS U01 FD001418HHS | U.S. Food and Drug Administration (FDA) U01FDU001418
6 · The paper itself

Abstract

Oxford Nanopore sequencing is one of the high-throughput sequencing technologies that facilitates the reconstruction of metagenome-assembled genomes (MAGs). This study aimed to assess the potential of long-read assembly algorithms in Oxford Nanopore sequencing to enhance the MAG-based identification of bacterial pathogens using both simulated and mock communities. Simulated communities were generated to mimic those on fresh spinach and in surface water. Long reads were produced using R9.4.1+SQK-LSK109 and R10.4 + SQK-LSK112, with 0.5, 1, and 2 million reads. The simulated bacterial communities included multidrug-resistant

Indexed as

AlgorithmsGenome, BacterialHigh-Throughput Nucleotide SequencingMetagenomeNanopore SequencingBacteriaComputational BiologyMetagenomicsPseudomonas aeruginosaSalmonella entericalong readmetagenome-assembled genomemetagenome assemblynanopore chemistryOxford Nanopore sequencingpathogen identificationsequencing depth

Identifiers

PMID38687063
PMCPMC11237517

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.