Evidence map›Paper›PMID 38685086›Full record

ArticleCancer cell international2024

Exosome-mediated secretion of miR-127-3p regulated by RAB27A accelerates metastasis in renal cell carcinoma.

Dae Hyun Song, Jong Sil Lee, Jeong-Hee Lee, Dong Chul Kim, Jung Wook Yang, Min Hye Kim, Ji Min Na, Hyun-Kyung Cho, Jiyun Yoo, Hyo Jung An

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer cell international, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 9 citations in OpenAlex.

  1. Rab27: Molecular switch of tumor exosome secretion (Review).International journal of molecular medicine · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Dae Hyun SongDepartment of Pathology, Gyeongsang National University Changwon Hospital, Changwon, South Korea.
Jong Sil LeeInstitute of Medical Sciences, Gyeongsang National University, Jinju, South Korea.
Jeong-Hee LeeInstitute of Medical Sciences, Gyeongsang National University, Jinju, South Korea.
Dong Chul KimInstitute of Medical Sciences, Gyeongsang National University, Jinju, South Korea.
Jung Wook YangInstitute of Medical Sciences, Gyeongsang National University, Jinju, South Korea.
Min Hye KimDepartment of Pathology, Gyeongsang National University Hospital, Jinju, South Korea.
Ji Min NaDepartment of Pathology, Gyeongsang National University Hospital, Jinju, South Korea.
Hyun-Kyung ChoInstitute of Medical Sciences, Gyeongsang National University, Jinju, South Korea.
Jiyun YooDivision of Applied Life Science (BK21 Plus) and Research Institute of Life Sciences, Gyeongsang National University, Jinju, South Korea.
Hyo Jung AnDepartment of Pathology, Gyeongsang National University Changwon Hospital, Changwon, South Korea. ariel2020@naver.com.
Gyeongsang National University · KRGyeongsang National University Hospital · KR

Funding

lnstitute of Health Sciences of Gyeongsang National University IHS GNU-2021-02National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIT) RS- 2022-00166664
6 · The paper itself

Abstract

backgroundThe exosome-mediated extracellular secretion of miRNAs occurs in many cancers, and RAB27A is a potent regulator of exosome secretion. For metastatic renal cell carcinoma (RCC), this study examines the mechanisms of cancer metastasis via the RAB27A-regulated secretion of specific miRNAs.

methodsRAB27A knockdown (KD) and overexpressing (OE) RCC cells were used to examine cell migration and adhesion. The particle counts and sizes of exosomes in RAB27A OE cells were analyzed using Exoview, and those of intraluminal vesicles (ILV) and multivesicular bodies (MVB) were measured using an electron microscope. Analysis of RNA sequences, protein-protein interaction networks, and the competing endogenous RNA (ceRNA) network were used to identify representative downregulated miRNAs that are likely to undergo cargo-sorting into exosomes and subsequent secretion. A molecular beacon of miR-137-3p, one of the most representatively downregulated genes with a fold change of 339, was produced, and its secretion was analyzed using Exoview. RAB27A OE and control cells were incubated in an exosome-containing media to determine the uptake of tumor suppressor miRNAs that affect cancer cell metastasis.

resultsMigration and cell adhesion were higher in RAB27A OE cells than in RAB27A KD cells. Electron microscopy revealed that the numbers of multivesicular bodies and intraluminal vesicles per cell were higher in RAB27A OE cells than in control cells, suggesting their secretion. The finding revealed that miR-127-3p was sorted into exosomes and disposed of extracellularly. Protein-protein interaction analysis revealed MYCN to be the most significant hub for RAB27A-OE RCC cells. ceRNA network analysis revealed that MAPK4 interacted strongly with miR-127-3p.

conclusionThe disposal of miR-127-3p through exosome secretion in RAB27A overexpressing cells may not inhibit the MAPK pathway to gain metastatic potential by activating MYCN. The exosomes containing miRNAs are valuable therapeutic targets for cancer treatment.

Identifiers

PMID38685086
PMCPMC11057152
OpenAlexW4396238674

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.