Evidence map›Paper›PMID 38684225›Full record

ArticleACS synthetic biology2024

Dynamic Multiplexed Control and Modeling of Optogenetic Systems Using the High-Throughput Optogenetic Platform, Lustro.

Zachary P Harmer, Jaron C Thompson, David L Cole, Ophelia S Venturelli, Victor M Zavala, Megan N McClean

Abstract read
In one paragraph

Article in ACS synthetic biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Characterizing Optogenetic Tools for Use in Synthetic Gene Circuits.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  2. Review
  3. Article
  4. Review
  5. Protein design accelerates the development and application of optogenetic tools.Computational and structural biotechnology journal · 2025
    Review
  6. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Zachary P HarmerDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.
Jaron C ThompsonDepartment of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.
David L ColeDepartment of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.
Ophelia S VenturelliDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.ORCID 0000-0003-2200-1963
Victor M ZavalaDepartment of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.ORCID 0000-0002-5744-7378
Megan N McCleanDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.ORCID 0000-0002-1141-4802

Funding

Institutional Training in the Genomic SciencesT32HG002760 · NHGRI · UNIVERSITY OF WISCONSIN-MADISON · PI Qiongshi Lu · 2003 to 2026
$17.7M
Elucidating the network design principles of biological signal processingR35GM128873 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Megan N McClean · 2018 to 2026
$4.0M
Elucidating the molecular and ecological design principles of stability and assembly of the human gut microbiotaR35GM124774 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Ophelia Venturelli · 2017 to 2026
$2.9M
Model-guided design of next-generation bacterial therapeutics to treat cardiovascular diseaseR01EB030340 · NIBIB · UNIVERSITY OF WISCONSIN-MADISON · PI REY, FEDERICO E, ROMERO, PHILIP ANTHONY · 2020 to 2023
$2.8M
NHGRI NIH HHS T32 HG002760NIBIB NIH HHS R01 EB030340NIGMS NIH HHS R35 GM124774NIGMS NIH HHS R35 GM128873
6 · The paper itself

Abstract

The ability to control cellular processes using optogenetics is inducer-limited, with most optogenetic systems responding to blue light. To address this limitation, we leverage an integrated framework combining Lustro, a powerful high-throughput optogenetics platform, and machine learning tools to enable multiplexed control over blue light-sensitive optogenetic systems. Specifically, we identify light induction conditions for sequential activation as well as preferential activation and switching between pairs of light-sensitive split transcription factors in the budding yeast,

Indexed as

Bayes TheoremOptogeneticsSaccharomyces cerevisiaeHigh-Throughput Screening AssaysLightMachine LearningSynthetic BiologyTranscription FactorsTranscription Factorsautomationhigh throughputmachine learningMoClomodelingmultiplexingneural networkoptogeneticssynthetic transcription factorsyeast

Identifiers

PMID38684225
PMCPMC11106771

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.