Evidence map›Paper›PMID 38683335›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Mega-Barcoding Projects: Delivering National DNA Barcoding Initiatives for Plants.

Brandon S Whitley, Zhao Li, Laura Jones, Natasha de Vere

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Brandon S WhitleyNatural History Museum of Denmark, University of Copenhagen, Copenhagen, Denmark.
Zhao LiNatural History Museum of Denmark, University of Copenhagen, Copenhagen, Denmark.
Laura JonesNational Botanic Garden of Wales, Llanarthne, UK.
Natasha de VereNatural History Museum of Denmark, University of Copenhagen, Copenhagen, Denmark. natasha.de.vere@snm.ku.dk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plant DNA barcoding has a multitude of applications ranging from species detection and biomonitoring to investigating ecological networks and checking food quality. The ability to accurately identify species, using DNA barcoding, depends on the quality and comprehensiveness of the reference library that is used. This chapter describes how to create plant reference libraries using the rbcL, matK, and ITS2 DNA barcode regions. It covers the creation of species lists, the collection of specimens from the field and herbarium, DNA extraction, PCR amplification, and DNA sequencing. This methodology gives special attention to using samples from herbaria, as they represent important collections of easily accessible, taxonomically verified plant material.

Indexed as

DNA Barcoding, TaxonomicDNA, PlantPlantsGene LibraryPolymerase Chain ReactionSequence Analysis, DNADNA, PlantDigitizationDNA barcodingDNA extractionExtended specimen collectionHerbarium specimensITS2matKOpen accessPCRPlant collectionPlantsrbcLSpecies identification

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.