Evidence map›Paper›PMID 38683314›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Simple, Robust Invertebrate DNA Barcoding: Chelex-Based DNA Extraction and Optimized COI Amplification.

Jennifer Hackett, Sharon Pepenella, Cristina Fernandez Marco, Louise Bodt, Lina Ruiz Grajales, Jeffry Petracca, Justin Burke, Allison Mayle, Bruce Nash, David A Micklos

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jennifer HackettCold Spring Harbor Laboratory DNA Learning Center, Cold Spring Harbor, NY, USA. hackett@cshl.eduu.
Sharon PepenellaCold Spring Harbor Laboratory DNA Learning Center, Cold Spring Harbor, NY, USA.
Cristina Fernandez MarcoCold Spring Harbor Laboratory DNA Learning Center, Cold Spring Harbor, NY, USA.
Louise BodtCommittee on Evolutionary Biology, University of Chicago & Integrative Research Center, Field Museum of Natural History, Chicago, IL, USA.
Lina Ruiz GrajalesDepartment of Biological Sciences, Columbia University, New York, NY, USA.
Jeffry PetraccaCold Spring Harbor Laboratory DNA Learning Center, Cold Spring Harbor, NY, USA.
Justin BurkeCold Spring Harbor Laboratory DNA Learning Center, Cold Spring Harbor, NY, USA.
Allison MayleCold Spring Harbor Laboratory DNA Learning Center, Cold Spring Harbor, NY, USA.
Bruce NashCold Spring Harbor Laboratory DNA Learning Center, Cold Spring Harbor, NY, USA.
David A MicklosCold Spring Harbor Laboratory DNA Learning Center, Cold Spring Harbor, NY, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chelex-based DNA extractions are well suited for student DNA barcoding research because they are simple, safe, and inexpensive and can be performed without specialized laboratory equipment, allowing them to be performed in classrooms or at home. Extracted DNA is stable in Chelex solution for at least a week at ambient temperature, allowing collection of DNA samples from remote students. These extractions provide quality DNA for many taxa and are optimal for barcoding invertebrates, especially in combination with novel cytochrome c oxidase I (COI) primer cocktails and PCR cycling conditions.

Indexed as

DNA Barcoding, TaxonomicElectron Transport Complex IVPolymerase Chain ReactionAnimalsDNAInvertebratesDNAElectron Transport Complex IVAntBiologyChelexCOICourse-based undergraduate researchCUREDNA barcodingDNA extractionEducationHigh school researchInvertebratePCRRemote learning

Identifiers

PMID38683314

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.