Evidence map›Paper›PMID 38682696›Full record

ArticleBiology open2024

Arf GTPase-Activating proteins ADAP1 and ARAP1 regulate incorporation of CD63 in multivesicular bodies.

Kasumi Suzuki, Yoshitaka Okawa, Sharmin Akter, Haruki Ito, Yoko Shiba

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology open, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kasumi SuzukiGraduate course of Biological Sciences, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate University, 020-8551, Morioka, Japan.
Yoshitaka OkawaGraduate course of Biological Sciences, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate University, 020-8551, Morioka, Japan.
Sharmin AkterGraduate course of Biological Sciences, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate University, 020-8551, Morioka, Japan.
Haruki ItoBiological Sciences Course, Faculty of Science and Engineering, Iwate University, 020-8551, Morioka, Japan.
Yoko ShibaGraduate course of Biological Sciences, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate University, 020-8551, Morioka, Japan.ORCID 0000-0001-8229-541X

Funding

Iwate UniversityIwate University: Iwate Daigaku
6 · The paper itself

Abstract

Arf GTPase-activating proteins (ArfGAPs) mediate the hydrolysis of GTP bound to ADP-ribosylation factors. ArfGAPs are critical for cargo sorting in the Golgi-to-ER traffic. However, the role of ArfGAPs in sorting into intralumenal vesicles (ILVs) in multivesicular bodies (MVBs) in post-Golgi traffic remains unclear. Exosomes are extracellular vesicles (EVs) of endosomal origin. CD63 is an EV marker. CD63 is enriched ILVs in MVBs of cells. However, the secretion of CD63 positive EVs has not been consistent with the data on CD63 localization in MVBs, and how CD63-containing EVs are formed is yet to be understood. To elucidate the mechanism of CD63 transport to ILVs, we focused on CD63 localization in MVBs and searched for the ArfGAPs involved in CD63 localization. We observed that ADAP1 and ARAP1 depletion inhibited CD63 localization to enlarged endosomes after Rab5Q79L overexpression. We tested epidermal growth factor (EGF) and CD9 localization in MVBs. We observed that ADAP1 and ARAP1 depletion inhibited CD9 localization in enlarged endosomes but not EGF. Our results indicate ADAP1 and ARAP1, regulate incorporation of CD63 and CD9, but not EGF, in overlapped and different MVBs. Our work will contribute to distinguish heterogenous ILVs and exosomes by ArfGAPs.

Indexed as

Adaptor Proteins, Signal TransducingGTPase-Activating ProteinsMultivesicular BodiesTetraspanin 30ADP-Ribosylation FactorsCarrier ProteinsEndosomesHeLa CellsHumansNerve Tissue ProteinsProtein TransportADAP1 protein, humanAdaptor Proteins, Signal TransducingADP-Ribosylation FactorsARAP1 protein, humanCarrier ProteinsCD63 protein, humanGTPase-Activating ProteinsNerve Tissue ProteinsTetraspanin 30ArfGAPCD63ExosomeExtracellular vesiclesIntralumenal vesiclesMultivesicular bodies

Identifiers

PMID38682696
PMCPMC11103404

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.