Evidence map›Paper›PMID 38682378›Full record

ArticleBiomacromolecules2024

Thermally Robust Solvent-Free Liquid Polyplexes for Heat-Shock Protection and Long-Term Room Temperature Storage of Therapeutic Nucleic Acids.

Yiyan Chen, Xiaoyan Lin, Xuhan Liu, Yifan Liu, Liem Bui-Le, Anna K Blakney, Jonathan Yeow, Yunqing Zhu, Molly M Stevens, Robin J Shattock and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomacromolecules, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yiyan ChenDepartment of Chemical Engineering, Imperial College London, Exhibition Road, London SW7 2AZ, U.K.
Xiaoyan LinDepartment of Chemical Engineering, Imperial College London, Exhibition Road, London SW7 2AZ, U.K.
Xuhan LiuDepartment of Chemical Engineering, Imperial College London, Exhibition Road, London SW7 2AZ, U.K.
Yifan LiuDepartment of Chemical Engineering, Imperial College London, Exhibition Road, London SW7 2AZ, U.K.
Liem Bui-LeDepartment of Chemical Engineering, Imperial College London, Exhibition Road, London SW7 2AZ, U.K.
Anna K BlakneyDepartment of Infectious Disease, Imperial College London, Norfolk Place, London W2 1NY, U.K.ORCID 0000-0002-5812-9689
Jonathan YeowDepartment of Materials, Department of Bioengineering, and Institute of Biomedical Engineering at Imperial College London, Prince Consort Rd, SW7 2AZ London, South Kensington, U.K.ORCID 0000-0003-3709-5149
Yunqing ZhuSchool of Materials Science and Engineering, Tongji University, Shanghai 200092, China.ORCID 0000-0002-2557-003X
Molly M StevensDepartment of Materials, Department of Bioengineering, and Institute of Biomedical Engineering at Imperial College London, Prince Consort Rd, SW7 2AZ London, South Kensington, U.K.ORCID 0000-0002-7335-266X
Robin J ShattockDepartment of Infectious Disease, Imperial College London, Norfolk Place, London W2 1NY, U.K.
Rongjun ChenDepartment of Chemical Engineering, Imperial College London, Exhibition Road, London SW7 2AZ, U.K.ORCID 0000-0002-8133-5472
Alex P S BroganDepartment of Chemistry, King's College London, 7 Trinity Street, London SE1 1DB, U.K.ORCID 0000-0002-8361-6649
Jason P HallettDepartment of Chemical Engineering, Imperial College London, Exhibition Road, London SW7 2AZ, U.K.ORCID 0000-0003-3431-2371

Funding

Medical Research Council MR/R015651/1
6 · The paper itself

Abstract

Nucleic acid therapeutics have attracted recent attention as promising preventative solutions for a broad range of diseases. Nonviral delivery vectors, such as cationic polymers, improve the cellular uptake of nucleic acids without suffering the drawbacks of viral delivery vectors. However, these delivery systems are faced with a major challenge for worldwide deployment, as their poor thermal stability elicits the need for cold chain transportation. Here, we demonstrate a biomaterial strategy to drastically improve the thermal stability of DNA polyplexes. Importantly, we demonstrate long-term room temperature storage with a transfection efficiency maintained for at least 9 months. Additionally, extreme heat shock studies show retained luciferase expression after heat treatment at 70 °C. We therefore provide a proof of concept for a platform biotechnology that could provide long-term room temperature storage for temperature-sensitive nucleic acid therapeutics, eliminating the need for the cold chain, which in turn would reduce the cost of distributing life-saving therapeutics worldwide.

Indexed as

DNAHeat-Shock ResponseHot TemperatureHumansPolymersTemperatureTransfectionDNAPolymers

Identifiers

PMID38682378
PMCPMC11094731

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.