Evidence map›Paper›PMID 38679918›Full record

ArticleJournal of the American Society for Mass Spectrometry2024

Mass Spectrometry Reveals Molecular Effects of Citrulline Supplementation during Bone Fracture Healing in a Rat Model.

Sylvia Nauta, Johannes Greven, Martijn Hofman, Ronny Mohren, Dennis M Meesters, Diana Möckel, Twan Lammers, Frank Hildebrand, Tiffany Porta Siegel, Eva Cuypers and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Society for Mass Spectrometry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Trial
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Sylvia NautaDivision of Imaging Mass Spectrometry, Maastricht MultiModal Molecular Imaging (M4i) Institute, Maastricht University, 6229ER Maastricht, The Netherlands.
Johannes GrevenDepartment of Orthopedics, Trauma and Reconstructive Surgery, University Hospital RWTH Aachen, 52074 Aachen, Germany.
Martijn HofmanCenter of Musculoskeletal Surgery, Bonifatius Hospital Lingen, 49808 Lingen, Germany.
Ronny MohrenDivision of Imaging Mass Spectrometry, Maastricht MultiModal Molecular Imaging (M4i) Institute, Maastricht University, 6229ER Maastricht, The Netherlands.
Dennis M MeestersDepartment of Genetics & Cell Biology, Maastricht University, 6229ER Maastricht, The Netherlands.
Diana MöckelDepartment of Nanomedicine and Theranostics, Institute for Experimental Molecular Imaging, RWTH Aachen University Clinic, 52074 Aachen, Germany.
Twan LammersDepartment of Nanomedicine and Theranostics, Institute for Experimental Molecular Imaging, RWTH Aachen University Clinic, 52074 Aachen, Germany.ORCID 0000-0002-1090-6805
Frank HildebrandDepartment of Orthopedics, Trauma and Reconstructive Surgery, University Hospital RWTH Aachen, 52074 Aachen, Germany.
Tiffany Porta SiegelDivision of Imaging Mass Spectrometry, Maastricht MultiModal Molecular Imaging (M4i) Institute, Maastricht University, 6229ER Maastricht, The Netherlands.ORCID 0000-0001-5454-1863
Eva CuypersDivision of Imaging Mass Spectrometry, Maastricht MultiModal Molecular Imaging (M4i) Institute, Maastricht University, 6229ER Maastricht, The Netherlands.ORCID 0000-0002-6411-2461
Ron M A HeerenDivision of Imaging Mass Spectrometry, Maastricht MultiModal Molecular Imaging (M4i) Institute, Maastricht University, 6229ER Maastricht, The Netherlands.ORCID 0000-0002-6533-7179
Martijn PoezeDivision of Traumasurgery, Department of Surgery, Maastricht University Medical Center, 6229HX Maastricht, The Netherlands.ORCID 0000-0001-5005-1285

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bone fracture healing is a complex process in which specific molecular knowledge is still lacking. The citrulline-arginine-nitric oxide metabolism is one of the involved pathways, and its enrichment via citrulline supplementation can enhance fracture healing. This study investigated the molecular effects of citrulline supplementation during the different fracture healing phases in a rat model. Microcomputed tomography (μCT) was applied for the analysis of the fracture callus formation. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) and liquid-chromatography tandem mass spectrometry (LC-MS/MS) were used for lipid and protein analyses, respectively. μCT analysis showed no significant differences in the fracture callus volume and volume fraction between the citrulline supplementation and control group. The observed lipid profiles for the citrulline supplementation and control group were distinct for the different fracture healing stages. The main contributing lipid classes were phosphatidylcholines (PCs) and lysophosphatidylcholines (LPCs). The changing effect of citrulline supplementation throughout fracture healing was indicated by changes in the differentially expressed proteins between the groups. Pathway analysis showed an enhancement of fracture healing in the citrulline supplementation group in comparison to the control group via improved angiogenesis and earlier formation of the soft and hard callus. This study showed the molecular effects on lipids, proteins, and pathways associated with citrulline supplementation during bone fracture healing, even though no effect was visible with μCT.

Indexed as

CitrullineFracture HealingRats, Sprague-DawleyTandem Mass SpectrometryX-Ray MicrotomographyAnimalsBony CallusChromatography, LiquidDietary SupplementsDisease Models, AnimalLysophosphatidylcholinesMalePhosphatidylcholinesRatsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationCitrullineLysophosphatidylcholinesPhosphatidylcholines

Identifiers

PMID38679918
PMCPMC11157653

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.