Evidence map›Paper›PMID 38679676›Full record

ArticleScientific reports2024

Genetic interaction between TTG2 and AtPLC1 reveals a role for phosphoinositide signaling in a co-regulated suite of Arabidopsis epidermal pathways.

Aleah Goldberg, Patrick O'Connor, Cassandra Gonzalez, Mason Ouren, Luis Rivera, Noor Radde, Michael Nguyen, Felipe Ponce-Herrera, Alan Lloyd, Antonio Gonzalez

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PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Aleah GoldbergThe Freshman Research Initiative, The University of Texas at Austin, Austin, TX, 78712, USA.
Patrick O'ConnorThe Freshman Research Initiative, The University of Texas at Austin, Austin, TX, 78712, USA.
Cassandra GonzalezThe Freshman Research Initiative, The University of Texas at Austin, Austin, TX, 78712, USA.
Mason OurenThe Freshman Research Initiative, The University of Texas at Austin, Austin, TX, 78712, USA.
Luis RiveraThe Freshman Research Initiative, The University of Texas at Austin, Austin, TX, 78712, USA.
Noor RaddeThe Freshman Research Initiative, The University of Texas at Austin, Austin, TX, 78712, USA.
Michael NguyenThe Freshman Research Initiative, The University of Texas at Austin, Austin, TX, 78712, USA.
Felipe Ponce-HerreraThe Freshman Research Initiative, The University of Texas at Austin, Austin, TX, 78712, USA.
Alan LloydDepartment of Molecular Biosciences and The Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway, Austin, TX, 78712, USA.
Antonio GonzalezDepartment of Molecular Biosciences and The Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway, Austin, TX, 78712, USA. antonio.gonzalez@austin.utexas.edu.

Funding

Howard Hughes Medical Institute 52006985, 52008124
6 · The paper itself

Abstract

The TTG2 transcription factor of Arabidopsis regulates a set of epidermal traits, including the differentiation of leaf trichomes, flavonoid pigment production in cells of the inner testa (or seed coat) layer and mucilage production in specialized cells of the outer testa layer. Despite the fact that TTG2 has been known for over twenty years as an important regulator of multiple developmental pathways, little has been discovered about the downstream mechanisms by which TTG2 co-regulates these epidermal features. In this study, we present evidence of phosphoinositide lipid signaling as a mechanism for the regulation of TTG2-dependent epidermal pathways. Overexpression of the AtPLC1 gene rescues the trichome and seed coat phenotypes of the ttg2-1 mutant plant. Moreover, in the case of seed coat color rescue, AtPLC1 overexpression restored expression of the TTG2 flavonoid pathway target genes, TT12 and TT13/AHA10. Consistent with these observations, a dominant AtPLC1 T-DNA insertion allele (plc1-1D) promotes trichome development in both wild-type and ttg2-3 plants. Also, AtPLC1 promoter:GUS analysis shows expression in trichomes and this expression appears dependent on TTG2. Taken together, the discovery of a genetic interaction between TTG2 and AtPLC1 suggests a role for phosphoinositide signaling in the regulation of trichome development, flavonoid pigment biosynthesis and the differentiation of mucilage-producing cells of the seed coat. This finding provides new avenues for future research at the intersection of the TTG2-dependent developmental pathways and the numerous molecular and cellular phenomena influenced by phospholipid signaling.

Indexed as

Arabidopsis ProteinsGene Expression Regulation, PlantPhosphoinositide Phospholipase CPlant EpidermisSignal TransductionTranscription FactorsArabidopsisFlavonoidsMutationPhenotypePhosphatidylinositolsSeedsTrichomesArabidopsis ProteinsFlavonoidsPhosphatidylinositolsPhosphoinositide Phospholipase CPLC3 protein, ArabidopsisTranscription FactorsTTG2 protein, ArabidopsisAtPLC1Lipid signalingMBWPhosphoinositidesProanthocyanidinsSeed coatTrichomesTTG2

Identifiers

PMID38679676
PMCPMC11056374

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