Evidence map›Paper›PMID 38678249›Full record

ArticleBMC veterinary research2024

An improved system to generate recombinant canine distemper virus.

Huai Cheng, Hewei Zhang, Huayun Zhang, Huanchang Cai, Min Liu, Mingen Yu, Meihua Xiang, Shubo Wen, Jingqiang Ren

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Huai Cheng *Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, China.
Hewei Zhang *College of Food and Drugs, Luoyang Polytechnic, Luo Yang, China.
Huayun ZhangWenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, China.
Huanchang CaiWenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, China.
Min LiuWenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, China.
Mingen YuResearch and Development Department, Hangzhou Goodhere Biotechnology Co., Ltd, Hangzhou, China.
Meihua XiangResearch and Development Department, Hangzhou Goodhere Biotechnology Co., Ltd, Hangzhou, China.
Shubo WenPreventive Veterinary Laboratory, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China. wen0516@126.com.
Jingqiang RenWenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, China. rjq207@163.com.
Wenzhou University · CNHangzhou DAC Biotech (China) · CNInner Mongolia University for Nationalities · CN

Funding

The Core Technical Public Welfare Project of Luoyang No. 2202034AThe Youth Growth Technology Project of Jilin Provincial Science and Technology Development Program No. 20210508019RQ
6 · The paper itself

Abstract

backgroundCanine distemper virus (CDV) is a pathogen with the capability of cross-species transmission. It has crossed the species barrier to infect many other species, and its host range is expanding. The reverse genetic platform, a useful tool for scientific research, allows the generation of recombinant viruses from genomic cDNA clones in vitro.

methodsTo improve the reverse genetic system of CDV, a plasmid containing three independent expression cassettes was constructed for co-expression of the N, P, and L genes and then transfected with a full-length cDNA clone of CDV into Vero cells.

resultsThe results indicated that the established rescue system has the advantages of being more convenient, easy to control the transfection ratio, and high rescue efficiency compared with the conventional reverse genetics system.

conclusionThis method not only reduces the number of transfection plasmids, but also improves the rescue efficiency of CDV, which could provide a reference for the recovery of other morbilliviruses.

Indexed as

Distemper Virus, CaninePlasmidsAnimalsChlorocebus aethiopsDistemperDNA, ComplementaryReverse GeneticsTransfectionVero CellsDNA, ComplementaryCanine distemper virusMinirepliconNegative-sense RNARecombinant virusReverse genetic system

Identifiers

PMID38678249
PMCPMC11055280
OpenAlexW4395691527

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.