Evidence map›Paper›PMID 38676851›Full record

ArticleMarine biotechnology (New York, N.Y.)2024

Targeting TtVgR via siRNA Knockdown Elicits Ovarian Cell Death in the Tri-spine Horseshoe Crab.

Kianann Tan, Xiaowan Ma, Boyu Su, Chen Zhan, Xin Yang, Khor Waiho, Leong-Seng Lim, Kit Yue Kwan

Abstract read
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In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

Kianann TanGuangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou City, 535011, Guangxi, China.
Xiaowan MaKey Laboratory of Tropical Marine Ecosystem and Bioresource, Ministry of Natural Resources, Fourth Institute of Oceanography, Beihai, 536000, Guangxi, China.
Boyu SuGuangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou City, 535011, Guangxi, China.
Chen ZhanGuangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou City, 535011, Guangxi, China.
Xin YangGuangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou City, 535011, Guangxi, China.
Khor WaihoHigher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, 21030, Kuala Nerus City, Terengganu, Malaysia.
Leong-Seng LimBorneo Marine Research Institute, Universiti Malaysia Sabah, Kota Kinabalu City, Sabah, Malaysia.
Kit Yue KwanGuangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou City, 535011, Guangxi, China. kityuekwan@bbgu.edu.cn.
Beibu Gulf University · CNMinistry of Natural Resources · RWUniversiti Malaysia Terengganu · MYUniversiti of Malaysia Sabah · MY

Funding

High-level Talent Scientific Research Start-Up Fund Project of Beibu Gulf University 2022KYQD10Key Laboratory of Tropical Marine Ecosystem and Bioresource, Ministry of Natural Resources 2021QN05Marine Science Program for Guangxi First-Class Discipline, Beibu Gulf University DRA002, TRA001National Natural Science Foundation of China 42366006
6 · The paper itself

Abstract

The vitellogenin present in the bloodstream undergoes internalization into developing oocytes through the vitellogenin receptor (VgR), a process mediated by receptor-mediated endocytosis. VgR plays a crucial role in facilitating the accumulation of vitellogenin and the maturation of oocytes. In this study, we characterized a Tachypleus tridentatus vitellogenin receptor (TtVgR) gene from the tri-spine horseshoe crab, revealing a length of 1956 bp and encoding 652 amino acid residues with 12 exons. TtVgR has a molecular weight of 64.26 kDa and an isoelectric point of 5.95. Predictions indicate 85 phosphorylation sites and 7 glycosylation sites within TtVgR. Transcriptional analysis demonstrated specific expression of TtVgR in the ovary and yellow connective tissue. TtVgR was identified and distributed in the plasma membrane of oocytes. The siRNA-mediated TtVgR knockdown significantly reduced the transcriptional activity of TtVgR. This depletion induced excessive ROS production, resulting in DNA damage in ovarian primary cells. TUNEL and flow cytometry analyses confirmed ovarian cell apoptosis following TtVgR knockdown, indicating DNA damage in ovarian primary cells. These findings underscore the importance of TtVgR in ovarian cell development, suggesting its potential involvement in vitellogenesis and oocyte maturation. This knowledge may inform innovative breeding strategies and contribute to the sustainable management and conservation of the tri-spine horseshoe crab.

Indexed as

ApoptosisHorseshoe CrabsOvaryReceptors, Cell SurfaceRNA, Small InterferingAmino Acid SequenceAnimalsEgg ProteinsFemaleGene Knockdown TechniquesOocytesReactive Oxygen SpeciesEgg ProteinsReactive Oxygen SpeciesReceptors, Cell SurfaceRNA, Small Interferingvitellogenin receptorCell deathFunctional analysissiRNA knockdownTri-spine horseshoe crabVitellogenin receptor

Identifiers

PMID38676851
OpenAlexW4395704158

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.