Evidence map›Paper›PMID 38676737›Full record

ArticleBioprocess and biosystems engineering2024

Identification of hyperthermophilic D-allulose 3-epimerase from Thermotoga sp. and its application as a high-performance biocatalyst for D-allulose synthesis.

Ji-Dong Shen, Bao-Ping Xu, Te-Li Yu, Yong-Xiang Fei, Xue Cai, Liang-Gang Huang, Li-Qun Jin, Zhi-Qiang Liu, Yu-Guo Zheng

Abstract read
PubMed Publisher
In one paragraph

Article in Bioprocess and biosystems engineering, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
3.2field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Ji-Dong ShenThe National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
Bao-Ping XuThe National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
Te-Li YuThe National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
Yong-Xiang FeiThe National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
Xue CaiThe National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
Liang-Gang HuangThe National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
Li-Qun JinThe National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
Zhi-Qiang LiuThe National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China. microliu@zjut.edu.cn.
Yu-Guo ZhengThe National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
Zhejiang University of Technology · CN

Funding

Key R&D Program of Zhejiang 2023C01113National Key Research and Development Program of China 2022YFC2105400
6 · The paper itself

Abstract

D-Allulose 3-epimerase (DAE) is a vital biocatalyst for the industrial synthesis of D-allulose, an ultra-low calorie rare sugar. However, limited thermostability of DAEs hinders their use at high-temperature production. In this research, hyperthermophilic TI-DAE (Tm = 98.4 ± 0.7 ℃) from Thermotoga sp. was identified via in silico screening. A comparative study of the structure and function of site-directed saturation mutagenesis mutants pinpointed the residue I100 as pivotal in maintaining the high-temperature activity and thermostability of TI-DAE. Employing TI-DAE as a biocatalyst, D-allulose was produced from D-fructose with a conversion rate of 32.5%. Moreover, TI-DAE demonstrated excellent catalytic synergy with glucose isomerase CAGI, enabling the one-step conversion of D-glucose to D-allulose with a conversion rate of 21.6%. This study offers a promising resource for the enzyme engineering of DAEs and a high-performance biocatalyst for industrial D-allulose production.

Indexed as

ThermotogaBacterial ProteinsBiocatalysisCarbohydrate EpimerasesEnzyme StabilityFructoseHot TemperatureMutagenesis, Site-DirectedRacemases and EpimerasesBacterial ProteinsCarbohydrate EpimerasesFructosepsicoseRacemases and EpimerasesCoupled catalysisD-AlluloseD-Allulose 3-epimeraseHyperthermophilic enzymeMolecular dynamics simulation

Identifiers

PMID38676737
OpenAlexW4395691184

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.