Evidence map›Paper›PMID 38675887›Full record

ArticleViruses2024

The mRNA Vaccine Expressing Single and Fused Structural Proteins of Porcine Reproductive and Respiratory Syndrome Induces Strong Cellular and Humoral Immune Responses in BalB/C Mice.

Luoyi Zhou, Ashenafi Kiros Wubshet, Jiangrong Zhang, Shitong Hou, Kaishen Yao, Qiuyi Zhao, Junfei Dai, Yongsheng Liu, Yaozhong Ding, Jie Zhang and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
4.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Luoyi ZhouCollege of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066600, China.ORCID 0009-0008-3029-5977
Ashenafi Kiros WubshetState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China.ORCID 0000-0002-9008-2075
Jiangrong ZhangState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China.
Shitong HouState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China.
Kaishen YaoZhaoqing Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Zhaoqing 526000, China.
Qiuyi ZhaoCollege of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066600, China.
Junfei DaiState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China.
Yongsheng LiuCollege of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066600, China.
Yaozhong DingState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China.ORCID 0000-0003-3684-8217
Jie ZhangCollege of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066600, China.
Yuefeng SunState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China.
Lanzhou Veterinary Research Institute · CNHebei Normal University of Science and Technology · CNZhaoqing University · CN

Funding

Hebei Province Key R & D Program 21322913DHebei Provincial Key Laboratory of Preventive Veterinary Medicine post-performance subsidy 20567621HKey Development and Research Foundation of Gansu 20YF3NA005National key R&D Program of China-Mongolia Intergovernmental International S&T Innovation Cooperation Program 2023YFE0199900Natural Science Foundation of Hebei Province Project Funds C2022407019The Project Funds of Hebei Normal University of Science and Technology Nos. 2022YB009 and 2022YB010
6 · The paper itself

Abstract

PRRS is a viral disease that profoundly impacts the global swine industry, causing significant economic losses. The development of a novel and effective vaccine is crucial to halt the rapid transmission of this virus. There have been several vaccination attempts against PRRSV using both traditional and alternative vaccine design development approaches. Unfortunately, there is no currently available vaccine that can completely control this disease. Thus, our study aimed to develop an mRNA vaccine using the antigens expressed by single or fused PRRSV structural proteins. In this study, the nucleotide sequence of the immunogenic mRNA was determined by considering the antigenicity of structural proteins and the stability of spatial structure. Purified GP5 protein served as the detection antigen in the immunological evaluation. Furthermore, cellular mRNA expression was detected by immunofluorescence and western blotting. In a mice experiment, the Ab titer in serum and the activation of spleen lymphocytes triggered by the antigen were detected by ELISA and ICS, respectively. Our findings demonstrated that both mRNA vaccines can significantly stimulate cellular and humoral immune responses. More specifically, the GP5-mRNA exhibited an immunological response that was similar to that of the commercially available vaccine when administered in high doses. To conclude, our vaccine may show promising results against the wild-type virus in a natural host.

Indexed as

Antibodies, ViralImmunity, CellularImmunity, HumoralMice, Inbred BALB CmRNA VaccinesPorcine Reproductive and Respiratory SyndromePorcine respiratory and reproductive syndrome virusViral Envelope ProteinsViral VaccinesAnimalsFemaleMiceRNA, MessengerSwineViral Structural ProteinsAntibodies, Viralglycoprotein 5, PRRSVmRNA VaccinesRNA, MessengerViral Envelope ProteinsViral Structural ProteinsViral VaccinesICSin vitro transcriptionmRNA vaccinePRRSPRRSVstructural protein

Identifiers

PMID38675887
PMCPMC11054013
OpenAlexW4393356582

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.