ArticleInternational journal of molecular sciences2024
Sperm Chromatin Dispersion Test Detects Sperm DNA Fragmentation Mainly Associated with Unviable Spermatozoa and Underestimates the Values with Respect to TUNEL Assay.
Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 2 of them syntheses that pooled it.
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The trial behind it
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Who cites it
10 citing papers in PubMed, 2 syntheses or guidelines pooled it, 10 citations in OpenAlex.
- Phthalates as the silent saboteurs of male fertility via changes in semen quality: a systematic review.Reproductive biology and endocrinology : RB&E · 2026Pooled it
- Impact of short abstinence versus testicular sperm on sperm DNA fragmentation: a systematic review and meta-analysis.Archives of gynecology and obstetrics · 2024Pooled it
- Rho-kinase inhibition reduces DNA fragmentation in human sperm cryopreservation.Asian journal of andrology · 2026Article
- Clustering of sperm by chromatin integrity features reveals breed and monthly effects, as well as a link between double-strand DNA breaks and litter size in pigs.BMC veterinary research · 2026Article
- Decoding Infertility: the Epigenetic Influence of HDACs on Reproductive Function.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- Prolonged exposure to polyvinylpyrrolidone heightens DNA breaks in human sperm.Scientific reports · 2026Article
- Expert-Annotated Optical Microscopy Images of Human Sperm for Detection and DNA Fragmentation Assessment.Scientific data · 2025Article
- The Utility of Sperm DNA Fragmentation as a Diagnostic Tool for Male Infertility and Its Predictive Value for Assisted Reproductive Technology Outcomes.International journal of molecular sciences · 2025Article
- Pathogenesis of testicular dysfunction in diabetes: exploring the mechanism and therapeutic interventions.Journal of assisted reproduction and genetics · 2025Review
- Association of Sperm DNA Fragmentation and Intracytoplasmic Sperm Injection Outcomes in an Age-adjusted Cohort: A Retrospective Cohort Study.Journal of human reproductive sciencesArticle
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Several clinical laboratories assess sperm DNA fragmentation (sDF) in addition to semen analysis in male infertility diagnosis. Among tests evaluating sDF, TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) and SCD (Sperm Chromatin Dispersion) are widely used. Our lab developed a modified version of TUNEL (TUNEL/PI) able to distinguish two sperm populations (PI Brighter and PI Dimmer) differently associated with sperm viability and reproductive outcomes. The aim of this study was to compare sDF levels detected by SCD and TUNEL/PI in the semen samples from 71 male subjects attending our Andrology Laboratory. Our results demonstrate that SCD is less sensitive in determining sDF compared to TUNEL/PI. The statistically significant positive correlation found between sDF evaluated by SCD and PI Dimmer (consisting of all dead spermatozoa) suggests that SCD mainly detects sDF in unviable spermatozoa. We confirmed that most spermatozoa detected by SCD are unviable by performing SCD after incubation in hypo-osmotic medium to discriminate viable and unviable cells in 52 samples. Such results might explain the lower ability of this test in discriminating couples having successful ART outcomes demonstrated in published metanalyses. Overall, our results indicate that SCD is less sensitive in evaluating sDF for diagnostic purposes.
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Registered trials
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