ArticleInternational journal of molecular sciences2024
Defining the Most Potent Osteoinductive Culture Conditions for MC3T3-E1 Cells Reveals No Implication of Oxidative Stress or Energy Metabolism.
Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 5 citations in OpenAlex.
- Coriander Honey Accelerates Human Osteoblast Differentiation and Matrix Mineralization via Intracellular CaPharmaceuticals (Basel, Switzerland) · 2026Article
- Friedelin Enhances Gelatin/Nanohydroxyapatite Scaffold Performance by Inhibiting Osteoclastogenesis and Promoting Osteogenesis.ACS omega · 2026Article
- Silk cryogel and electrospun scaffold characterization for bone-tendon interface applications.Frontiers in bioengineering and biotechnology · 2026Article
- Insights into the potential role of BMSCs-exo delivered USP14 on SIRT1 deubiquitination in Staphylococcus aureus-induced model of osteomyelitis.Journal of orthopaedic surgery and research · 2025Article
- Synthesis and Osteoinductive Properties of Nanosized Lithium-Modified Calcium-Organic Frameworks.Materials (Basel, Switzerland) · 2025Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
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Abstract
The MC3T3-E1 preosteoblastic cell line is widely utilised as a reliable in vitro system to assess bone formation. However, the experimental growth conditions for these cells hugely diverge, and, particularly, the osteogenic medium (OSM)'s composition varies in research studies. Therefore, we aimed to define the ideal culture conditions for MC3T3-E1 subclone 4 cells with regard to their mineralization capacity and explore if oxidative stress or the cellular metabolism processes are implicated. Cells were treated with nine different combinations of long-lasting ascorbate (Asc) and β-glycerophosphate (βGP), and osteogenesis/calcification was evaluated at three different time-points by qPCR, Western blotting, and bone nodule staining. Key molecules of the oxidative and metabolic pathways were also assessed. It was found that sufficient mineral deposition was achieved only in the 150 μg.mL
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