ArticleParasites & vectors2024
Seroprevalence and molecular detection of Toxoplasma gondii and Neospora caninum in beef cattle and goats in Hunan province, China.
Article in Parasites & vectors, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Seroprevalence ofVeterinary sciences · 2026Article
- Review
- Genetic Diversity of Neospora Caninum in Palestinian Cattle and Sheep: Insights From Nc-5 Gene Detection and MS10 Microsatellite Analysis.Veterinary medicine and science · 2026Article
- Population genetic structure of zoonoticScience in One Health · 2026Article
- Serological investigation ofOpen veterinary journal · 2026Article
- Seromolecular and histopathological study on Toxoplasma gondii infection in ruminants in Aswan, Egypt.BMC veterinary research · 2025Article
- A novel TaqMan probe-based pentaplex qPCR assay for the simultaneous detection of five pathogenic protozoans.Scientific reports · 2025Article
- Serological and Molecular Detection of Toxoplasma gondii Among Slaughtered Domestic Ruminants in Gondar Town, Northwest Ethiopia.Veterinary medicine and science · 2025Article
- Molecular Prevalence and Genotyping ofAnimals : an open access journal from MDPI · 2025Article
- Serological and molecular investigation ofOpen veterinary journal · 2025Article
- Review
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10 authors.
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Abstract
backgroundToxoplasma gondii and Neospora caninum are closely related protozoan parasites that are considered important causes of abortion in livestock, causing huge economic losses. Hunan Province ranks 12th in the production of beef and mutton in China. However, limited data are available on the seroprevalence, risk factors and molecular characterization of T. gondii and N. caninum in beef cattle and goats in Hunan province, China.
methodsSera of 985 beef cattle and 1147 goats were examined for the presence of specific antibodies against T. gondii using indirect hemagglutination test (IHAT) and anti-N. caninum IgG using competitive-inhibition enzyme-linked immunoassay assay (cELISA). Statistical analysis of possible risk factors was performed using PASW Statistics. Muscle samples of 160 beef cattle and 160 goats were examined for the presence of T. gondii DNA (B1 gene) and N. caninum DNA (Nc-5 gene) by nested PCR. The B1 gene-positive samples were genotyped at 10 genetic markers using the multilocus nested PCR-RFLP (Mn-PCR-RFLP).
resultsSpecific IgG against T. gondii were detected in 8.3% (82/985) and 13.3% (153/1147) and against N. caninum in 2.1% (21/985) and 2.0% (23/1147) of the beef cattle and goats, respectively. Based on statistical analysis, the presence of cats, semi-intensive management mode and gender were identified as significant risk factors for T. gondii infection in beef cattle. Age was a significant risk factor for T. gondii infection in goats (P < 0.05), and age > 3 years was a significant risk factor for N. caninum infection in beef cattle (P < 0.05). PCR positivity for T. gondii was observed in three beef samples (1.9%; 3/160) and seven chevon samples (4.4%; 7/160). Genotyping of PCR positive samples identified one to be ToxoDB#10. The N. caninum DNA was observed in one beef sample (0.6%; 1/160) but was negative in all chevon samples.
conclusionsTo our knowledge, this is the first large-scale serological and molecular investigation of T. gondii and N. caninum and assessment of related risk factors in beef cattle and goats in Hunan Province, China. The findings provide baseline data for executing prevention and control of these two important parasites in beef cattle and goats in China.
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