Evidence map›Paper›PMID 38671404›Full record

ArticleBMC biotechnology2024

Multiprotein collagen/keratin hydrogel promoted myogenesis and angiogenesis of injured skeletal muscles in a mouse model.

Atieh Rezaei Namjoo, Ayla Hassani, Hassan Amini, Fateme Nazaryabrbekoh, Sepideh Saghati, Mohammad Ali Ebrahimi Saadatlou, Ali Baradar Khoshfetrat, Nafiseh Didar Khosrowshahi, Reza Rahbarghazi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.6field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Atieh Rezaei NamjooStem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St, Golgasht St, Tabriz, Iran.
Ayla HassaniChemical Engineering Faculty, Sahand University of Technology, Tabriz, 51335-1996, Iran.
Hassan AminiDepartment of General and Vascular Surgery, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Fateme NazaryabrbekohStem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St, Golgasht St, Tabriz, Iran.
Sepideh SaghatiDepartment of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Mohammad Ali Ebrahimi Saadatlou *Department of Basic Sciences, College of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran. ebrahimi@iaut.ac.ir.
Ali Baradar KhoshfetratChemical Engineering Faculty, Sahand University of Technology, Tabriz, 51335-1996, Iran.
Nafiseh Didar KhosrowshahiChemical Engineering Faculty, Sahand University of Technology, Tabriz, 51335-1996, Iran.
Reza Rahbarghazi *Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St, Golgasht St, Tabriz, Iran. rezarahbardvm@gmail.com.
Tabriz University of Medical Sciences · IRSahand University of Technology · IRIslamic Azad University of Tabriz · IR

Funding

Tabriz University of Medical Sciences 65618
6 · The paper itself

Abstract

Volumetric loss is one of the challenging issues in muscle tissue structure that causes functio laesa. Tissue engineering of muscle tissue using suitable hydrogels is an alternative to restoring the physiological properties of the injured area. Here, myogenic properties of type I collagen (0.5%) and keratin (0.5%) were investigated in a mouse model of biceps femoris injury. Using FTIR, gelation time, and rheological analysis, the physicochemical properties of the collagen (Col)/Keratin scaffold were analyzed. Mouse C2C12 myoblast-laden Col/Keratin hydrogels were injected into the injury site and histological examination plus western blotting were performed to measure myogenic potential after 15 days. FTIR indicated an appropriate interaction between keratin and collagen. The blend of Col/Keratin delayed gelation time when compared to the collagen alone group. Rheological analysis revealed decreased stiffening in blended Col/Keratin hydrogel which is favorable for the extrudability of the hydrogel. Transplantation of C2C12 myoblast-laden Col/Keratin hydrogel to injured muscle tissues led to the formation of newly generated myofibers compared to cell-free hydrogel and collagen groups (p < 0.05). In the C2C12 myoblast-laden Col/Keratin group, a low number of CD31

Indexed as

KeratinsMuscle DevelopmentMuscle, SkeletalAngiogenesisAnimalsCell LineCollagenDisease Models, AnimalHydrogelsMaleMiceMyoblastsNeovascularization, PhysiologicTissue EngineeringTissue ScaffoldsCollagenHydrogelsKeratinsCollagen/keratin hydrogelMouseMyogenic propertiesTissue engineeringVolumetric loss

Identifiers

PMID38671404
PMCPMC11055224
OpenAlexW4395676313

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.