ArticleNature communications2024
Probing the surface charge of condensates using microelectrophoresis.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 25 citations in OpenAlex.
- Seeing the Chemistry of Biomolecular Condensates: In Situ Mapping of Composition and Water Content.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Sustained formation movement and destruction of coacervates in a waste-free system driven by electricity.Nature communications · 2026Article
- Electrostatic bimodality of human cytosolic DNA sensor cGAS-dsDNA condensates revealed by millisecond flashing electrophoretic separation.Nature communications · 2026Article
- In Situ Quantification of Bacterial Surface Charge at the Single-Cell Level for Modeling Transport under Electric Fields.Environmental science & technology · 2026Article
- A high-throughput, flow cytometry approach to measure phase behavior and exchange in biomolecular condensates.Nature communications · 2026Article
- Biomolecular Condensates Can Induce Local Membrane Potentials.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Controlling interfacial protein adsorption, desorption and aggregation in biomolecular condensates.Nature communications · 2025Article
- Automated navigation of condensate phase behavior with active machine learning.Nature communications · 2025Article
- Amino acids bind to phase-separating proteins and modulate biomolecular condensate stability and dynamics.Nature communications · 2025Article
- Microscopy-based techniques for studying the material properties of biomolecular condensates in the cellular environment.Biophysical reviews · 2025Review
- Selective Ion Binding and Uptake Shape the Microenvironment of Biomolecular Condensates.Journal of the American Chemical Society · 2025Article
- Controlling Multiphase Coacervate Wetting and Self-Organization by Interfacial Proteins.Journal of the American Chemical Society · 2025Article
- Stabilization of Condensate Interfaces Using Dynamic Protein Insertion.Journal of the American Chemical Society · 2025Article
- Induction and Manipulation of Biomolecular Condensates Through Spatially Heterogeneous Solution Conditions.Chembiochem : a European journal of chemical biology · 2025Article
- Resilient Membranized Coacervates Formed through Spontaneous Wrapping of Heat-Destabilized Lipid Bilayers around Coacervate Droplets.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Live-cell quantification reveals viscoelastic regulation of synapsin condensates by α-synuclein.Science advances · 2025Article
- Lipid packing and cholesterol content regulate membrane wetting and remodeling by biomolecular condensates.Nature communications · 2025Article
- Selective peptide bond formation via side chain reactivity and self-assembly of abiotic phosphates.Nature communications · 2025Article
- Molecular determinants of condensate composition.Molecular cell · 2025Review
- Biomolecular Condensates can Induce Local Membrane Potentials.bioRxiv : the preprint server for biology · 2024Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Biomolecular condensates play an important role in cellular organization. Coacervates are commonly used models that mimic the physicochemical properties of biomolecular condensates. The surface of condensates plays a key role in governing molecular exchange between condensates, accumulation of species at the interface, and the stability of condensates against coalescence. However, most important surface properties, including the surface charge and zeta potential, remain poorly characterized and understood. The zeta potential of coacervates is often measured using laser doppler electrophoresis, which assumes a size-independent electrophoretic mobility. Here, we show that this assumption is incorrect for liquid-like condensates and present an alternative method to study the electrophoretic mobility of coacervates and in vitro condensate models by microelectrophoresis and single-particle tracking. Coacervates have a size-dependent electrophoretic mobility, originating from their fluid nature, from which a well-defined zeta potential is calculated. Interestingly, microelectrophoresis measurements reveal that polylysine chains are enriched at the surface of polylysine/polyaspartic acid complex coacervates, which causes the negatively charged protein ɑ-synuclein to adsorb and accumulate at the interface. Addition of ATP inverts the surface charge, displaces ɑ-synuclein from the surface and may help to suppress its interface-catalyzed aggregation. Together, these findings show how condensate surface charge can be measured and altered, making this microelectrophoresis platform combined with automated single-particle tracking a promising characterization technique for both biomolecular condensates and coacervate protocells.
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