Evidence map›Paper›PMID 38668967›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

DNA Isolation Long-Read Genomic Sequencing in Ctenophores.

David Moraga Amador, Andrea B Kohn, Yelena Bobkova, Nedka G Panayotova, Leonid L Moroz

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

David Moraga AmadorInterdisciplinary Center for Biotechnology (ICBR), University of Florida, Gainesville, FL, USA. moraga@ufl.edu.
Andrea B KohnWhitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, USA.
Yelena BobkovaWhitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, USA.
Nedka G PanayotovaInterdisciplinary Center for Biotechnology (ICBR), University of Florida, Gainesville, FL, USA.
Leonid L MorozWhitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, USA. moroz@whitney.ufl.edu.

Funding

Neuron-SELEX: Development of neuron-specific nanoscale toolkits for single-cell recognitionR01NS114491 · NINDS · UNIVERSITY OF FLORIDA · PI MOROZ, LEONID L · 2020 to 2024
$2.0M
NINDS NIH HHS R01 NS114491
6 · The paper itself

Abstract

Long-read sequencing has proven the necessity for high-quality genomic assemblies of reference species, including enigmatic ctenophores. Obtaining high-molecular-weight genomic DNA is pivotal to this process and has proven highly problematic for many species. Here, we discuss different methodologies for gDNA isolation and present a protocol for isolating gDNA for several members of the phylum Ctenophora. Specifically, we describe a Pacific Biosciences library construction method used in conjunction with gDNA isolation methods that have proven successful in obtaining high-quality genomic assemblies in ctenophores.

Indexed as

CtenophoraDNAGenomicsSequence Analysis, DNAAnimalsGene LibraryGenomeHigh-Throughput Nucleotide SequencingDNABasal metazoansBeroeBolinopsisCtenophoraEvolutionGenomeLong-read sequencingMnemiopsisPacBio sequencingPleurobrachia

Identifiers

PMID38668967

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.