Evidence map›Paper›PMID 38668917›Full record

ArticleMarine biotechnology (New York, N.Y.)2024

Genome Analysis of Multiple Polysaccharide-Degrading Bacterium Microbulbifer thermotolerans HB226069: Determination of Alginate Lyase Activity.

Xue Li, Miao Yang, Kunlian Mo, Yonghua Hu, Hanjie Gu, Dongmei Sun, Shixiang Bao, Huiqin Huang

Abstract read
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In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xue LiCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, 163000, Heilongjiang, China.
Miao YangInstitute of Tropical Bioscience and Biotechnology, Hainan Institute for Tropical Agricultural Resources, CATAS, Haikou, 571101, Hainan, China.
Kunlian MoInstitute of Tropical Bioscience and Biotechnology, Hainan Institute for Tropical Agricultural Resources, CATAS, Haikou, 571101, Hainan, China.
Yonghua HuInstitute of Tropical Bioscience and Biotechnology, Hainan Institute for Tropical Agricultural Resources, CATAS, Haikou, 571101, Hainan, China.
Hanjie GuInstitute of Tropical Bioscience and Biotechnology, Hainan Institute for Tropical Agricultural Resources, CATAS, Haikou, 571101, Hainan, China.
Dongmei SunCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, 163000, Heilongjiang, China. sdmlzw@126.com.
Shixiang BaoInstitute of Tropical Bioscience and Biotechnology, Hainan Institute for Tropical Agricultural Resources, CATAS, Haikou, 571101, Hainan, China. baoshixiang@itbb.org.cn.
Huiqin HuangInstitute of Tropical Bioscience and Biotechnology, Hainan Institute for Tropical Agricultural Resources, CATAS, Haikou, 571101, Hainan, China. huanghuiqin@itbb.org.cn.

Funding

Central Public-interest Scientific Institution Basal Research Fund from Chinese Government 1630052019014Hainan Provincial Department of Science and Technology 323QN278Ministry of Agriculture and Rural Affairs of the People's Republic of China NFZX2021
6 · The paper itself

Abstract

Polysaccharide-degrading bacteria are key participants in the global carbon cycle and algal biomass recycling. Herein, a polysaccharide lyase-producing strain HB226069 was isolated from Sargassum sp. from Qingge Port, Hainan, China. Results of the phylogenetic of the 16S rRNA gene and genotypic analysis indicated that the isolate should be classified as Microbulbifer thermotolerans. The whole genome is a 4,021,337 bp circular chromosome with a G+C content of 56.5%. Analysis of the predicted genes indicated that strain HB226069 encoded 161 carbohydrate-active enzymes (CAZymes), and abundant putative enzymes involved in polysaccharide degradation were predicted, including alginate lyase, fucosidase, agarase, xylanase, cellulase, pectate lyase, amylase, and chitinase. Three of the putative polysaccharide lyases from PL7 and PL17 families were involved in alginate degradation. The alginate lyases of strain HB226069 showed the maximum activity of 117.4 U/mL at 50 °C, pH 7.0, and 0.05 M FeCl

Indexed as

Genome, BacterialPhylogenyPolysaccharide-LyasesAlginatesBacterial ProteinsBacteroidetesBase CompositionChinaPolysaccharidesRNA, Ribosomal, 16SSargassumAlginatesBacterial Proteinspoly(beta-D-mannuronate) lyasePolysaccharide-LyasesPolysaccharidesRNA, Ribosomal, 16SAlginate lyaseGenomeMicrobulbifer thermotoleransOligosaccharidePolysaccharide-degrading bacteria

Identifiers

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.