Evidence map›Paper›PMID 38668881›Full record

ArticlePlanta2024

Unraveling the role of PlARF2 in regulating deed formancy in Paeonia lactiflora.

Siyang Duan, Shixin Guan, Riwen Fei, Tianyi Sun, Xuening Kang, Rujie Xin, Wenhui Song, Xiaomei Sun

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In one paragraph

Article in Planta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Siyang DuanCollege of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Shixin GuanCollege of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Riwen FeiCollege of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Tianyi SunCollege of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Xuening KangCollege of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Rujie XinCollege of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Wenhui SongCollege of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Xiaomei SunCollege of Forestry, Shenyang Agricultural University, Shenyang, 110866, China. sxm7280@syau.edu.cn.

Funding

National Natural Science Foundation of China 31470696National Natural Science Foundation of China 32071814
6 · The paper itself

Abstract

MAIN

conclusionPlARF2 can positively regulate the seed dormancy in Paeonia lactiflora Pall. and bind the RY cis-element. Auxin, a significant phytohormone influencing seed dormancy, has been demonstrated to be regulated by auxin response factors (ARFs), key transcriptional modulators in the auxin signaling pathway. However, the role of this class of transcription factors (TFs) in perennials with complex seed dormancy mechanisms remains largely unexplored. Here, we cloned and characterized an ARF gene from Paeonia lactiflora, named PlARF2, which exhibited differential expression levels in the seeds during the process of seed dormancy release. The deduced amino acid sequence of PlARF2 had high homology with those of other plants and contained typical conserved Auxin_resp domain of the ARF family. Phylogenetic analysis revealed that PlARF2 was closely related to VvARF3 in Vitis vinifera. The subcellular localization and transcriptional activation assay showed that PlARF2 is a nuclear protein possessing transcriptional activation activity. The expression levels of dormancy-related genes in transgenic callus indicated that PlARF2 was positively correlated with the contents of PlABI3 and PlDOG1. The germination assay showed that PlARF2 promoted seed dormancy. Moreover, TF Centered Yeast one-hybrid assay (TF-Centered Y1H), electrophoretic mobility shift assay (EMSA) and dual-luciferase reporter assay analysis (Dual-Luciferase) provided evidence that PlARF2 can bind to the 'CATGCATG' motif. Collectively, our findings suggest that PlARF2, as TF, could be involved in the regulation of seed dormancy and may act as a repressor of germination.

Indexed as

Gene Expression Regulation, PlantPaeoniaPhylogenyPlant DormancyPlant ProteinsAmino Acid SequenceGerminationIndoleacetic AcidsPlant Growth RegulatorsPlants, Genetically ModifiedSeedsTranscription FactorsIndoleacetic AcidsPlant Growth RegulatorsPlant ProteinsTranscription FactorsAuxin response factorCis-elementPhylogenetic analysisSeed dormancyTranscription factor

Identifiers

PMID38668881

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.