ArticleeLife2024
Deep learning insights into the architecture of the mammalian egg-sperm fusion synapse.
Article in eLife, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
22 citing papers in PubMed, 22 citations in OpenAlex.
- WFS1 Deficiency Impairs PIAS4-Associated SUMOylation and Increases Ubiquitin-Mediated Spermatogenesis-Related Protein Degradation Leading to Testicular Male Infertility.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Molecular determinants of species-specific gamete adhesion triggering HAP2-mediated membrane fusion.Nature communications · 2026Article
- What will be the future of computational biology for macromolecules in the era of AI?PLoS computational biology · 2026Article
- Molecular interplay between sperm and oocyte: a narrative review.Human reproduction update · 2026Review
- Sperm surface protein disulfide isomerase ERp57 is crucial for mammalian fertilization but functions independently of IZUMO1.Cellular & molecular biology letters · 2026Article
- Characterization ofbioRxiv : the preprint server for biology · 2026Article
- Structure and inhibition of the sperm TMEM95-FIMP complex in mammalian fertilization.bioRxiv : the preprint server for biology · 2026Article
- The SPARK complex forms the molecular basis of vertebrate fertilization.bioRxiv : the preprint server for biology · 2026Article
- Breaking cellular boundaries: molecular mechanisms of tunneling nanotube formation and fusion.Biochemical Society transactions · 2026Review
- JUNO-coated beads as a functional assay to capture and characterize fertilization-competent human sperm.Human reproduction open · 2026Article
- PRSS38 Is a Novel Sperm Serine Protease Involved in Human and Mouse Fertilization.International journal of molecular sciences · 2025Article
- Spermatozoa lacking TEX51 display hypofertility and defects in morphology.Communications biology · 2025Article
- PRAMEY enhances sperm-egg binding and modulates epigenetic dynamics in bovine embryogenesis.Cell and tissue research · 2025Article
- An orphan gene is essential for efficient sperm entry into eggs in Drosophila melanogaster.Genetics · 2025Article
- Allosteric inhibition of the IZUMO1-JUNO fertilization complex by the naturally occurring antisperm antibody OBF13.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Reproductive health.eLife · 2025Article
- Acrosomal Matrix Proteins sp56 and AM67 and Gamete Adhesion in Mammals.Academia biology · 2025Article
- Immunolocalization and proteomic analyses of IZUMO1 in porcine spermatozoa.Frontiers in cell and developmental biology · 2025Article
- Computational insights in CD58 binding to ASFV CD2v and in silico optimization of nanobody designs against the interface.Open veterinary journal · 2025Article
- An orphan gene is essential for efficient sperm entry into eggs inbioRxiv : the preprint server for biology · 2024Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
A crucial event in sexual reproduction is when haploid sperm and egg fuse to form a new diploid organism at fertilization. In mammals, direct interaction between egg JUNO and sperm IZUMO1 mediates gamete membrane adhesion, yet their role in fusion remains enigmatic. We used AlphaFold to predict the structure of other extracellular proteins essential for fertilization to determine if they could form a complex that may mediate fusion. We first identified TMEM81, whose gene is expressed by mouse and human spermatids, as a protein having structural homologies with both IZUMO1 and another sperm molecule essential for gamete fusion, SPACA6. Using a set of proteins known to be important for fertilization and TMEM81, we then systematically searched for predicted binary interactions using an unguided approach and identified a pentameric complex involving sperm IZUMO1, SPACA6, TMEM81 and egg JUNO, CD9. This complex is structurally consistent with both the expected topology on opposing gamete membranes and the location of predicted N-glycans not modeled by AlphaFold-Multimer, suggesting that its components could organize into a synapse-like assembly at the point of fusion. Finally, the structural modeling approach described here could be more generally useful to gain insights into transient protein complexes difficult to detect experimentally.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.