Evidence map›Paper›PMID 38664610›Full record

ArticleBMC plant biology2024

GMOIT: a tool for effective screening of genetically modified crops.

Pu Zhou, Xuan Liu, Jingang Liang, Juanli Zhao, Yuqi Zhang, Dongmei Xu, Xiaying Li, Ziyan Chen, Zongyong Shi, Jianhua Gao

Open access · goldAbstract read
In one paragraph

Article in BMC plant biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Pu Zhou *Hou Ji Laboratory in Shanxi Province, College of Life Sciences, Shanxi Agricultural University, Jinzhong, 030801, Shanxi, China.
Xuan Liu *Hou Ji Laboratory in Shanxi Province, College of Life Sciences, Shanxi Agricultural University, Jinzhong, 030801, Shanxi, China.
Jingang Liang *Development Center for Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing, 100025, China.
Juanli ZhaoHou Ji Laboratory in Shanxi Province, College of Life Sciences, Shanxi Agricultural University, Jinzhong, 030801, Shanxi, China.
Yuqi ZhangCrops Ecological Environment Security Inspection and Supervision Center (Taiyuan), Ministry of Agriculture and Rural Affairs, Taigu, 030801, Shanxi, China.
Dongmei XuHou Ji Laboratory in Shanxi Province, College of Life Sciences, Shanxi Agricultural University, Jinzhong, 030801, Shanxi, China.
Xiaying LiDevelopment Center for Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing, 100025, China.
Ziyan ChenDevelopment Center for Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing, 100025, China.
Zongyong ShiHou Ji Laboratory in Shanxi Province, College of Life Sciences, Shanxi Agricultural University, Jinzhong, 030801, Shanxi, China. zongyongtg@sohu.com.
Jianhua GaoHou Ji Laboratory in Shanxi Province, College of Life Sciences, Shanxi Agricultural University, Jinzhong, 030801, Shanxi, China. jhgao@sxau.edu.cn.
Shanxi Agricultural University · CNMinistry of Agriculture and Rural Affairs · CN

Funding

the Central Government Guides the Local Science and Technology Development Fund Project YDZJSX20231B007The Science and Technology Innovation 2030 2022ZD040200802
6 · The paper itself

Abstract

backgroundAdvancement in agricultural biotechnology has resulted in increasing numbers of commercial varieties of genetically modified (GM) crops worldwide. Though several databases on GM crops are available, these databases generally focus on collecting and providing information on transgenic crops rather than on screening strategies. To overcome this, we constructed a novel tool named, Genetically Modified Organisms Identification Tool (GMOIT), designed to integrate basic and genetic information on genetic modification events and detection methods.

resultsAt present, data for each element from 118 independent genetic modification events in soybean, maize, canola, and rice were included in the database. Particularly, GMOIT allows users to customize assay ranges and thus obtain the corresponding optimized screening strategies using common elements or specific locations as the detection targets with high flexibility. Using the 118 genetic modification events currently included in GMOIT as the range and algorithm selection results, a "6 + 4" protocol (six exogenous elements and four endogenous reference genes as the detection targets) covering 108 events for the four crops was established. Plasmids pGMOIT-1 and pGMOIT-2 were constructed as positive controls or calibrators in qualitative and quantitative transgene detection.

conclusionsOur study provides a simple, practical tool for selecting, detecting, and screening strategies for a sustainable and efficient application of genetic modification.

Indexed as

Crops, AgriculturalGlycine maxOryzaPlants, Genetically ModifiedBrassica napusTransgenesZea maysBiotechnologyDatabaseGenetically modified cropsMulti-target plasmidsScreening strategyTransgene detection

Identifiers

PMID38664610
PMCPMC11044397
OpenAlexW4395463319

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.