Evidence map›Paper›PMID 38664194›Full record

ArticleNeurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics2024

AAV6 mediated Gsx1 expression in neural stem progenitor cells promotes neurogenesis and restores locomotor function after contusion spinal cord injury.

Zachary Finkel, Fatima Esteban, Brianna Rodriguez, Tanner Clifford, Adelina Joseph, Hani Alostaz, Mridul Dalmia, Juan Gutierrez, Matthew J Tamasi, Samuel Ming Zhang and 9 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.3field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 2 institutions in 1 country.

Zachary FinkelDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA.
Fatima EstebanDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA.
Brianna RodriguezDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA.
Tanner CliffordDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA.
Adelina JosephDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA.
Hani AlostazDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA.
Mridul DalmiaDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA.
Juan GutierrezDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA; University of California, Santa Barbara, CA 93106, USA.
Matthew J TamasiDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA.
Samuel Ming ZhangDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA.
Jonah SimoneDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA.
Hafize PetekciDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA.
Susmita NathDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA.
Miriam EscottDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA.
Shivam Kumar GargDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA.
Adam J GormleyDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA.
Suneel KumarDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA.
Sonia GulatiNeuroNovus Therapeutics Inc., 135 E 57th St., New York, NY 10022, USA.
Li CaiDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway, NJ 08854, USA; NeuroNovus Therapeutics Inc., 135 E 57th St., New York, NY 10022, USA. Electronic address: lcai@rutgers.edu.
Rutgers, The State University of New Jersey · USIo Therapeutics (United States) · US

Funding

UMDNJ TRAINING GRANT IN BIOTECHNOLOGYT32GM008339 · NIGMS · RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK · PI YARMUSH, MARTIN L · 1989 to 2019
$6.1M
IMSD at Rutgers - New Brunswick (Renewal)T32GM139804 · NIGMS · RUTGERS BIOMEDICAL AND HEALTH SCIENCES · PI Brian Daniels, JAMES H. MILLONIG · 2021 to 2026
$2.9M
Tuning multivalency for optimized ligand presentationR35GM138296 · NIGMS · RUTGERS, THE STATE UNIV OF N.J. · PI GORMLEY, ADAM JOSEPH · 2020 to 2024
$2.3M
NIGMS NIH HHS R35 GM138296NIGMS NIH HHS T32 GM008339NIGMS NIH HHS T32 GM139804
6 · The paper itself

Abstract

Genomic screened homeobox 1 (Gsx1 or Gsh1) is a neurogenic transcription factor required for the generation of excitatory and inhibitory interneurons during spinal cord development. In the adult, lentivirus (LV) mediated Gsx1 expression promotes neural regeneration and functional locomotor recovery in a mouse model of lateral hemisection spinal cord injury (SCI). The LV delivery method is clinically unsafe due to insertional mutations to the host DNA. In addition, the most common clinical case of SCI is contusion/compression. In this study, we identify that adeno-associated virus serotype 6 (AAV6) preferentially infects neural stem/progenitor cells (NSPCs) in the injured spinal cord. Using a rat model of contusion SCI, we demonstrate that AAV6 mediated Gsx1 expression promotes neurogenesis, increases the number of neuroblasts/immature neurons, restores excitatory/inhibitory neuron balance and serotonergic neuronal activity through the lesion core, and promotes locomotor functional recovery. Our findings support that AAV6 preferentially targets NSPCs for gene delivery and confirmed Gsx1 efficacy in clinically relevant rat model of contusion SCI.

Indexed as

DependovirusHomeodomain ProteinsLameness, AnimalNeural Stem CellsNeurogenesisSpinal InjuriesAnimalsDisease Models, AnimalFemaleMaleRecovery of FunctionSerotoninStem Cell TransplantationHomeodomain ProteinsSerotoninGene therapyGenomic screened homeobox 1 (Gsh1 or Gsx1)Neural regenerationNeural stem/progenitor cellNeurogenesisTraumatic spinal cord injury

Identifiers

PMID38664194
PMCPMC11452562
OpenAlexW4395113399

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.