Evidence map›Paper›PMID 38658747›Full record

ArticleNature2025

Whole-cortex in situ sequencing reveals input-dependent area identity.

Xiaoyin Chen, Stephan Fischer, Mara C P Rue, Aixin Zhang, Didhiti Mukherjee, Patrick O Kanold, Jesse Gillis, Anthony M Zador

Abstract read
In one paragraph

Article in Nature, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
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  9. Review
  10. Spatially tunable multiomic sequencing using light-driven combinatorial barcoding of molecules in tissues.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  11. Article
  12. Article
  13. Article
  14. Developing a Robust Multiround HCR-FISH Method Modified for Caenorhabditis elegans.Genes to cells : devoted to molecular & cellular mechanisms · 2026
    Article
  15. Post-Critical Period Transcriptional and Physiological Adaptations of Primary Sensory Cortex after Sensory Loss.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026
    Article
  16. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xiaoyin Chen *Allen Institute for Brain Science, Seattle, WA, USA. xiaoyin.chen@alleninstitute.org.ORCID 0000-0002-2807-6125
Stephan Fischer *Institut Pasteur, Université Paris Cité, Bioinformatics and Biostatistics Hub, Paris, France.ORCID 0000-0002-7034-4103
Mara C P Rue *Allen Institute for Brain Science, Seattle, WA, USA.
Aixin ZhangAllen Institute for Brain Science, Seattle, WA, USA.
Didhiti MukherjeeDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Patrick O KanoldDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.ORCID 0000-0002-7529-5435
Jesse GillisDepartment of Physiology, University of Toronto, Toronto, Ontario, Canada. jesse.gillis@utoronto.ca.ORCID 0000-0002-0936-9774
Anthony M ZadorCold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA. zador@cshl.edu.ORCID 0000-0002-8431-9136

Funding

Tool and Technology Development CoreU19MH114821 · NIMH · COLD SPRING HARBOR LABORATORY · PI MITRA, PARTHA PRATIM · 2017 to 2021
$64.5M
NEURONAL CIRCUITS AND PLASTICITY OF NEONATAL AUDITORY CORTEXR01DC009607 · NIDCD · UNIV OF MARYLAND, COLLEGE PARK · PI PATRICK O KANOLD · 2009 to 2026
$7.8M
SYNPLA: A scaleable method for monitoring circuit-specific learning-induced changes in synaptic strengthU01MH109113 · NIMH · COLD SPRING HARBOR LABORATORY · PI MALINOW, ROBERTO, ZADOR, ANTHONY M · 2015 to 2017
$2.9M
High throughput mapping of neuronal circuitry using DNA sequencingRF1MH114132 · NIMH · COLD SPRING HARBOR LABORATORY · PI ZADOR, ANTHONY M · 2017 to 2017
$2.7M
Unraveling the developmental logic of cortical long-range projections using in situ sequencing-based neuroanatomyDP2MH132940 · NIMH · ALLEN INSTITUTE · PI CHEN, XIAOYIN · 2022 to 2025
$2.6M
Revealing the transcriptomic basis of neuronal identity through functional meta-analysisR01MH113005 · NIMH · COLD SPRING HARBOR LABORATORY · PI GILLIS, JESSE · 2017 to 2021
$2.4M
High-throughput DNA sequencing method for probing the connectivity of neural circR01NS073129 · NINDS · COLD SPRING HARBOR LABORATORY · PI ZADOR, ANTHONY M · 2010 to 2014
$2.3M
CRCNS: Theory and experiment of neural circuit mapping by DNA sequencingR01DA036913 · NIDA · COLD SPRING HARBOR LABORATORY · PI KOULAKOV, ALEXEI, ZADOR, ANTHONY M · 2013 to 2017
$2.1M
Heuristics to evaluate biomedical and genomic knowledge bases for validityR01LM012736 · NLM · COLD SPRING HARBOR LABORATORY · PI GILLIS, JESSE · 2017 to 2020
$1.9M
Revealing the transcriptional basis of corticothalamic projections using in situ sequence-based neuroanatomyR01MH133181 · NIMH · ALLEN INSTITUTE · PI XIAOYIN CHEN · 2024 to 2026
$1.8M
Crossmodal plasticity in the auditory cortex in the precritical periodR21DC020560 · NIDCD · JOHNS HOPKINS UNIVERSITY · PI Didhiti Mukherjee · 2024 to 2026
$491k
NIDA NIH HHS R01 DA036913NIDCD NIH HHS R01 DC009607NIMH NIH HHS DP2 MH132940NIMH NIH HHS R01 MH113005NIMH NIH HHS R01 MH133181NIMH NIH HHS RF1 MH114132NIMH NIH HHS U01 MH109113NIMH NIH HHS U19 MH114821NINDS NIH HHS R01 NS073129NLM NIH HHS R01 LM012736
6 · The paper itself

Abstract

The cerebral cortex is composed of neuronal types with diverse gene expression that are organized into specialized cortical areas. These areas, each with characteristic cytoarchitecture

Indexed as

Cerebral CortexNeuronsTranscriptomeAnimalsAnimals, NewbornCluster AnalysisFemaleGene Expression ProfilingMaleMiceMice, Inbred C57BLProsencephalonSingle-Cell Analysis

Identifiers

PMID38658747
PMCPMC12589132

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.