Evidence map›Paper›PMID 38658418›Full record

ArticleArchives of virology2024

Comparison between different conditions for the incorporation of foreign proteins into Autographa californica multiple polyhedrovirus polyhedra for biotechnological purposes.

María Gabriela López, Cinthia Ayelén López, María José Gravisaco, Victoria Alfonso, Oscar Taboga

Erratum issuedAbstract readComparative Study
PubMed Publisher
In one paragraph

Article in Archives of virology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

María Gabriela LópezInstituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), De los Reseros y N. Repetto S/N, B1686IGC, Hurlingham, Buenos Aires, Argentina. lopez.mariag@inta.gob.ar.ORCID http://orcid.org/0000-0002-0560-7472
Cinthia Ayelén LópezInstituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), De los Reseros y N. Repetto S/N, B1686IGC, Hurlingham, Buenos Aires, Argentina.
María José GravisacoInstituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), De los Reseros y N. Repetto S/N, B1686IGC, Hurlingham, Buenos Aires, Argentina.
Victoria AlfonsoInstituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), De los Reseros y N. Repetto S/N, B1686IGC, Hurlingham, Buenos Aires, Argentina.
Oscar TabogaInstituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), De los Reseros y N. Repetto S/N, B1686IGC, Hurlingham, Buenos Aires, Argentina. taboga.oscaralberto@inta.gob.ar.

Funding

Fondo para la Investigación Científica y Tecnológica PICT 2601Instituto Nacional de Tecnología Agropecuaria 2019-PD-E5-I105-001
6 · The paper itself

Abstract

The occlusion bodies of Autographa californica multiple nucleopolyhedrovirus are proteinaceous formations with significant biotechnological potential owing to their capacity to integrate foreign proteins through fusion with polyhedrin, their primary component. However, the strategy for successful heterologous protein inclusion still requires further refinement. In this study, we conducted a comparative assessment of various conditions to achieve the embedding of recombinant proteins within polyhedra. Two baculoviruses were constructed: AcPHGFP (polh+), with GFP as a fusion to wild type (wt) polyhedrin and AcΔPHGFP (polh+), with GFP fused to a fragment corresponding to amino acids 19 to 110 of polyhedrin. These baculoviruses were evaluated by infecting Sf9 cells and stably transformed Sf9, Sf9POLH, and Sf9POLH

Indexed as

BiotechnologyNucleopolyhedrovirusesSpodopteraAnimalsCell LineGreen Fluorescent ProteinsOcclusion Bodies, ViralOcclusion Body Matrix ProteinsRecombinant Fusion ProteinsRecombinant ProteinsSf9 CellsGreen Fluorescent ProteinsOcclusion Body Matrix ProteinsRecombinant Fusion ProteinsRecombinant ProteinsAcMNPVBaculovirusGFPrOBs

Identifiers

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.