Evidence map›Paper›PMID 38658402›Full record

ArticleAnalytical and bioanalytical chemistry2024

Analysis of unsaturated alginate oligosaccharides using high-performance anion exchange chromatography coupled with mass spectrometry.

Jiajing Li, Menghui Sun, Xiao Song, Guangning Chen, Jinhang Zhou, Yaoguang Chang, Changhu Xue

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Article in Analytical and bioanalytical chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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3 · Its place in the literature

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2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Jiajing LiCollege of Food Science and Engineering, Ocean University of China, 1299 Sansha Road, Qingdao, 266404, China.
Menghui SunCollege of Food Science and Engineering, Ocean University of China, 1299 Sansha Road, Qingdao, 266404, China.
Xiao SongCollege of Food Science and Engineering, Ocean University of China, 1299 Sansha Road, Qingdao, 266404, China.
Guangning ChenCollege of Food Science and Engineering, Ocean University of China, 1299 Sansha Road, Qingdao, 266404, China.
Jinhang ZhouCollege of Food Science and Engineering, Ocean University of China, 1299 Sansha Road, Qingdao, 266404, China.
Yaoguang ChangCollege of Food Science and Engineering, Ocean University of China, 1299 Sansha Road, Qingdao, 266404, China. changyg@ouc.edu.cn.ORCID http://orcid.org/0000-0002-8234-7272
Changhu XueCollege of Food Science and Engineering, Ocean University of China, 1299 Sansha Road, Qingdao, 266404, China.

Funding

National Natural Science Foundation of China U22A20542
6 · The paper itself

Abstract

Alginate is a commercially important polysaccharide composed of mannuronic acid and its C5 differential isomer guluronic acid. Comprehensive research on alginate and alginate lyases requires efficient and precise analytical methods for alginate oligosaccharides. In this research, high-performance anion exchange chromatography (HPAEC) in parallel with pulsed amperometric detection (PAD) and mass spectrometry (MS) was applied to the analysis of oligosaccharides obtained by alginate lyase. By optimizing the chromatographic conditions including mobile phase concentration, flow rate, and elution gradient, the analysis of a single sample could be completed in 30 min. Seven unsaturated alginate oligosaccharides were separated and identified through their analysis time observed with PAD, including all structurally different unsaturated disaccharides and trisaccharides. The quantitative analysis of seven oligosaccharides was performed based on the quantitative capability of PAD. The method exhibited adequate linearity and precision parameters. All the calibration curves showed good linearity at least in the concentration range of 0.002 to 0.1 mg/mL. The HPAEC-PAD/MS method provides a general and efficient online method to analyze alginate oligosaccharides.

Indexed as

AlginatesMass SpectrometryOligosaccharidesChromatography, High Pressure LiquidChromatography, Ion ExchangeHexuronic AcidsLimit of DetectionPolysaccharide-LyasesAlginatesHexuronic AcidsOligosaccharidespoly(beta-D-mannuronate) lyasePolysaccharide-LyasesAlginate oligosaccharidesHPAEC-PAD/MSQuantitative analysisStructure analysis

Identifiers

PMID38658402

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.