Evidence map›Paper›PMID 38658334›Full record

ArticleThe Journal of veterinary medical science2024

Novel approach for detecting classical swine fever virus from swabs of wild boar cut tails using nested real-time PCR.

Kei Nabeshima, Takeo Kuriyama, Shun Takagi, Taro Sugimoto, Mayumi Yokoyama, Koichi Goka, Manabu Onuma

Open access · diamondAbstract read
In one paragraph

Article in The Journal of veterinary medical science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed, 1 pooled it
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 synthesis or guideline pooled it, 1 citations in OpenAlex.

  1. Pooled it
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Kei NabeshimaEcological Risk Assessment and Control Section Center for Environmental Biology and Ecosystem, National Institute for Environmental Studies, Ibaraki, Japan.
Takeo KuriyamaInstitute of Natural and Environmental Sciences, University of Hyogo, Hyogo, Japan.
Shun TakagiInstitute of Natural and Environmental Sciences, University of Hyogo, Hyogo, Japan.
Taro SugimotoInstitute of Natural and Environmental Sciences, University of Hyogo, Hyogo, Japan.
Mayumi YokoyamaInstitute of Natural and Environmental Sciences, University of Hyogo, Hyogo, Japan.
Koichi GokaEcological Risk Assessment and Control Section Center for Environmental Biology and Ecosystem, National Institute for Environmental Studies, Ibaraki, Japan.
Manabu OnumaEcological Risk Assessment and Control Section Center for Environmental Biology and Ecosystem, National Institute for Environmental Studies, Ibaraki, Japan.
National Institute for Environmental Studies · JPUniversity of Hyogo · JPHyogo University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We devised a method to detect the classical swine fever virus (CSFV) in tail-wiped swabs from wild boars. The CSFV gene in swabs was detected with high sensitivity using nested real-time polymerase chain reaction (PCR), which is a combination of reverse transcription-PCR (RT-PCR) and real-time PCR. We compared CSFV gene detection from boar tissue using the conventional and our tail-wiped swab method. The tail-wiped swab method showed sensitivity and specificity of 100% (26/26) and 98.8% (172/174), respectively compared to the conventional method. Thus, the swab-based CSFV detection method was considered to have detection sensitivity comparable to that of conventional methods. Additionally, we conducted surveillance for CSFV in wild boars on Awaji Island. CSFV was detected in 10.7% (45/420) of samples.

Indexed as

Classical Swine FeverClassical Swine Fever VirusReal-Time Polymerase Chain ReactionSensitivity and SpecificitySus scrofaAnimalsJapanReverse Transcriptase Polymerase Chain ReactionSwineTailclassical swine feverqPCRsampling methodwild boar

Identifiers

PMID38658334
PMCPMC11187583
OpenAlexW4395098057

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.